藥疹患者外周血淋巴細(xì)胞亞群的檢測及其意義-論文.pdf

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1、·1266·國際檢驗醫(yī)學(xué)雜志2014年5月第35卷第lO期IntJLabMed，May2014，Vo1．35，No．10·臨床檢驗研究論著·藥疹患者外周血淋巴細(xì)胞亞群的檢測及其意義譚飛，莫小輝，陳佳。，章楚光。，胡婷婷，吳飛，宋寧靜。，顧軍(1．第二軍醫(yī)大學(xué)附屬長海醫(yī)院皮膚科，上海200433；2．上海市皮膚病醫(yī)院中心實驗室，上海200443)摘要：目的分析藥疹患者外周血淋巴細(xì)胞亞群的變化。方法將18例初診藥疹患者作為藥疹組，根據(jù)致敏藥物的不同將其分為頭孢菌素組(一9)、青霉素組(”=5)及中藥組(”一4)，將2O例健康體檢者作為對照組。應(yīng)用流式細(xì)胞儀檢測其

2、外周血T淋巴細(xì)胞(CD3、CD3CD4、CD3CD8)、B淋巴細(xì)胞、自然殺傷細(xì)胞(NK)和自然殺傷T淋巴細(xì)胞(NKT)所占百分比及其絕對計數(shù)。結(jié)果藥疹組患者外周血T淋巴細(xì)胞(CD3、CD3CD4)、B淋巴細(xì)胞和NKT細(xì)胞所占百分比與對照組的差異有統(tǒng)計學(xué)意義(P<0．05)；藥疹組患者CD3CD8淋巴細(xì)胞所占百分比與對照組的差異無統(tǒng)計學(xué)意義(P>0．05)，而其T、B淋巴細(xì)胞亞群的絕對計數(shù)與對照組的差異有統(tǒng)計學(xué)意義(P

3、巴細(xì)胞亞群；流式細(xì)胞術(shù)；外周血DOI：10．3969／j．issn．1673—4130．2014．10．014文獻(xiàn)標(biāo)識碼：A文章編號：1673—4130(2O14)10—1266一o3DetectionoflymphocytesubsetsinperipheralbloodofpatientswithdrugeruptionanditssignificanceTanFei”，MoXiaohui，ChenJia。，ZhangChuguang，HuTingting，WuFei，SongNingjing，GuJun(1．DepartmentofDermatolog

4、y，ChanghaiHospitalAfJiliatedtotheSecondMilitaryMedicalUniversity，Shanghai200433，China；2．CentralLaboratory，ShanghaiDermatologyHospital，Shanghai200443，China)Abstract：ObjectiveToanalyzethechangesoflymphocytesubsetsinperipheralbloodofpatientswithdrugeruption．Methods18newlydiagnosedpati

5、entswereservedasthedrugeruptiongroup．a(chǎn)ndweresubdividedintocephalosporingroup(n一9)。penicillingroup(n一5)andChinesemedicinegroup(n一4)accordingtodifferentsensitizingdrugs．2Ohealthypeopleweretakenasthecontrolgroup．FlowcytometrywereutilizedtodetectthepercentagesandabsolutecountsofTlympho

6、cytes(CD3，CD3CD4andCD3CD8)，Blymphocytes，naturalkillercell(NK)andnaturalkillerTlymphocytes(NKT)intheirperipheralblood．ResultsDifferencesofpercentagesofTlymphocytes(CD3，CD3CD4)，Blymphocytes，NKTcellsbetweenthedrugeruptiongroupandthecontrolgroupshowedstatisticalsignificant(P<0．05)．Diff

7、erenceofpercentagesofCD3CD8lymphoeytesofpa—tientsbetweenthedrugeruptiongroupandthecontrolgroupdemonstratednostatisticalsignificant(P>O．05)，whilethatofabso—lutecountsofTandBlymphocytesofpatientswasstatisticalsignificantbetweenthedrugeruptiongroupandthecontrolgroup(P<0．05)．Conclusion

8、ThepercentagesofCD3，CD3CD4