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1、2010年第25卷第6期⑨JournalofTUSVo1.25No.62010囪圓甘氨酸對(duì)力竭性游泳小鼠抗體形成細(xì)胞的影響樊曉飛1,李良菊2,馬斌2,丁建霞2,成靜2摘要:探討甘氨酸補(bǔ)充對(duì)一次力竭性運(yùn)動(dòng)小鼠抗體形成細(xì)胞的影響。方法:將小鼠隨機(jī)分成力竭性游泳運(yùn)動(dòng)組(E),甘氨酸補(bǔ)充后力竭性游泳組(Gly+E),甘氨酸補(bǔ)充對(duì)照組(Gly),靜息正常對(duì)照組(S),每組8只。在Gly+E、Gly組補(bǔ)充甘氨酸,其余兩組給予生理鹽水,處理4周后,E組和Gly+E組均做力竭性游泳運(yùn)動(dòng)。實(shí)驗(yàn)前5天用SRBC免疫小鼠,
2、采用定量溶血測(cè)定法(QHS)測(cè)定小鼠脾臟內(nèi)抗體形成細(xì)胞溶解SRBC的能力;用微量凝集法測(cè)定小鼠血清中抗SRBC抗體的效價(jià);測(cè)定胸腺指數(shù)和脾臟指數(shù)。結(jié)果:與E組比較,GIy+E組的力竭性游泳運(yùn)動(dòng)時(shí)間顯著延長(zhǎng)(PO.05;脾臟指數(shù)各組間比較有非常顯
3、著性差異P<0.01。結(jié)論:甘氨酸預(yù)補(bǔ)充可提高力竭性游泳運(yùn)動(dòng)的最大操作能力;力竭性游泳運(yùn)動(dòng)降低血清中抗體效價(jià),抗體形成細(xì)胞亦減少,甘氨酸預(yù)補(bǔ)充可對(duì)抗這些變化。關(guān)鍵詞:甘氨酸;力竭性游泳;抗體;抗體形成細(xì)胞中圖分類號(hào):G804.23文獻(xiàn)標(biāo)識(shí)碼:A文章編號(hào):1005—0000(2010)06—0526—03InfluenceofGlycineonAntibodyCellsFormingofExhaustiveMiceFANXiaofei,LILian~iu,MABin,DINGJianxia,CHENGJ
4、in(1.Dept.ofPE,JiangsuUniversity,Zhenjiang212013,China;2.SchoolofMedicalandLaboratoryMedicine,JiangsuUniversity,Zhen—jiang212013,China)Abstract:Objective:Thisstudywastoexploretheinfluenceofglycinesupplementationonantibodyformingcellsforexhaustivephysica
5、lexertioninmice.Methods:Themicewererandomlydividedintofourgroups:anexhaustiveswimminggroup(E),agroupofexhaustiveswimmingafterglycinesupplemen-tation(Gly+E),andasedentarygroup(S),aglycinesupplementationandsedentarygroup(Gly),8animalseachgroup.Glycineands
6、alineweresupple-mentedrespectivelyintothemiceintheGly+EandEgroups,orthemiceintheothers,afterfourweeksbyintragastrieintubation.BoththemiceoftheEandtheGly+Eweresubjectedtoaboutofexhaustiveswimmingexercise.Thecontentsantibodyintheserumofmicewasanalyzedusin
7、gmicroagglutinationtest.Thecontentsantibodyformingcellsinthespleenofmicewereanalyzedusingquantitativehematolyticspectrophotometriedetermination(QHS).Thy—musindexandspleenindexofmiceweredetermined.Results:TheGIy+Egrouphadasignificantlylongertimeofexhaust
8、iveswimmingthantheE.ThecontentsantibodyinthesenlmweredecreasedintheEandSgroupscomparedtotheGIy+EandGLYgroups(P