rp-hplc同時(shí)測(cè)定鼠掌老鸛草中5種活性成分的含量

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1、RP-HPLC同時(shí)測(cè)定鼠掌老鸛草中5種活性成分的含量羅宏，尹海波（遼寧中醫(yī)藥大學(xué)藥學(xué)院，遼寧大連116600）[摘要]目的：建立HPLC法同時(shí)測(cè)定鼠掌老鸛草中沒食子酸,原兒茶酸,槲皮素,山奈素,山奈素-3,7-O-а-二鼠李糖苷的含量測(cè)定方法。方法：采用RP-HPLC法，AgilentTC-C18色譜柱(250mm*4.6mm)，0.4％磷酸水溶液-甲醇為流動(dòng)相梯度洗脫，流速0.8mL·min-1,檢測(cè)波長(zhǎng)為切換波長(zhǎng),(0-23min)波長(zhǎng)270nm;(23-55min)波長(zhǎng)370nm.柱溫30℃。結(jié)果：沒食

2、子酸,原兒茶酸,槲皮素,山奈素,山奈素-3,7-O-α-二鼠李糖苷的線性范圍為:0.192~1.732g.L-1(r=0.9997),0.016~0.256g.L-1(r=0.9999),0.004~0.039g.L-1(r=0.9999),0.004~0.041g.L-1(r=0.9999),0.2004~1.6032g.L-1(r=0.9998),平均回收率（n=3）分別為100.07%(RSD1.5%),99.55%(RSD1.7%),100.28%(RSD2.2%),99.99%(RSD1.8%),9

3、9.90%(RSD1.0%)。結(jié)論：該含量測(cè)定的方法快速、準(zhǔn)確、可靠、重復(fù)性好，可為藥材的質(zhì)量控制提供依據(jù)?！娟P(guān)鍵詞】鼠掌老鸛草；沒食子酸；原兒茶酸；槲皮素；山奈素；HPLCSimultaneousdeterminationoffiveactivecompoundsinGeraniumsibiricumL.byRP-HPLCLUOHong,YINHaibo(LiaoningUniversityofTraditionalChineseMedicine，Dalian116600，China)[Abstract]O

4、bjective:Todetermine5activecompoundsinGeraniumsibiricumL.,namelygallicacid,protocatechuinacid,quercetin,kaempferol-7-O-rhamnosideandkaemferitrin.Method:TheRP-HPLCmethodwasappliedandtheseparationwasperformedonanAgilentTC-C18analyticalcolumn(250mmХ4.6mm,5μm).

5、Themobilephasewasmethanol(A)-watercontaining0.4%H3PO4(B)withgradientelutionmodeattheflowrateof0.9mL?min.Thedetectionwassetat270nm(0-23min),370nm(23-55min),andthecolumntemperaturewas30℃.Result:ThelinerrangesofGallicacid,protocatechuinacid,quercetin,kaempfero

6、l-7-O-rhamnosideandkaemferitrinwere0.192~1.732g.L-1(r=0.9997),0.016~0.256g.L-1(r=0.9999),0.004~0.039g.L-1(r=0.9999),0.004~0.041g.L-1(r=0.9999),0.2004~1.6032g.L-1(r=0.9998).Theaveragerecoveries(n=3)were100.07%(RSD1.5%),99.55%(RSD1.7%),100.28%(RSD2.2%),99.99%

7、(RSD1.8%),99.90%(RSD1.0%).Conclusion:ThemethordissimpleandaccuratewithagoodreproducibilityandcanbeusedasaqualitycontrolmethordforGeraniumsibiricumL.ofdifferentsources.[Keywords]GeraniumsibiricumL.;Gallicacid;Protocatechuinacid;Quercetin;Kaempferol-7-O-rhamn

8、oside;HPLC老鸛草為我國傳統(tǒng)中藥，來源于牻牛兒苗科植物牻牛兒苗（ErodiumstephanianumWilld.）、老鸛草（GeraniumwilfordiiMaxim.）或野老鸛草(GeraniumcarolinianumL.)的干燥地上部分,收載于我國10版藥典，前者習(xí)稱”長(zhǎng)嘴老鸛草”，后兩者習(xí)稱”短嘴老鸛草”[1]。性辛、苦、平，歸肝、腎、脾經(jīng)，祛風(fēng)濕、通經(jīng)絡(luò)、主治筋骨酸痛、泄瀉痢疾