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1、YangHetong,TangWenhua,MaartenRyder,LiJishun,GuoYong,ZhouHongzi.2004.LectinsoffungalpathogensaspotentialtoolsinselectingpromisingbiologicalcontrolagentsofTrichodermaspp.J.ShandongUniversityofTechnology(Sci&Tech),18(6):15-23Lectinsoffungalpathogensaspotentialtoolsinselectingpromisingbiologicalcontro
2、lagentsofTrichodermaspp.YangHetong1,2,3,4,TangWenhua2,MaartenRyder3,LiJishun1,GuoYong1,ZhouHongzi1(1BiologyResearchInstituteofShandongAcademyofSciences,Jinan,250014;2Dept.PlantPathology,ChinaAgriculturalUniversity,Beijing,China,100094;3AdelaideLab,CSIROLandandWater,GlenOsmond,SA5064,AdelaideAustra
3、lia;4LifeScienceCollege,ShandongUniversityofTechnology,Zibo,255049)Abstract:Inthisarticle,lectinsof8pathogenstrainswereextractedandpurified.Bytestingagglutinationlectinswith19Trichodermaspp.srains,alllectinsfromfungiagglutinatedtheconidiaofTrichodermaspp.withdifferenttiters,exceptlectinfromAlterna
4、riasolaniMs.ResultindicatedthattheagglutinationofTrichodermaconidiabyfungallectinswasrelatedtotheireffectivenesscausedbythecorrespondingpathogens.StatisticalanalysisshowedsignificantcorrelationbetweentitersofFusariumlectinandtheeffectivenessofTrichodermaagainstthedisease.Theagglutinationtestmightb
5、eappliedtopredictpotentialeffectivenessofagivenTrichodermastrainagainstthecorrespondingdiseaseandthelectinscouldbeemployedinscreeningTrichodermasppasbiocontrolagentsefficiently.Keywords:lectins;pathogens;agglutination;TrichodermasppAsuitablemethodwascrucialforobtainingefficientbiocontrolmicrobiala
6、ntagonistsinsoilborneplantdiseasecontrol.Thestrategiesadoptedbymostresearchersatpresentinscreeningwerevarious(Songetal,1996),butthereweretwobasicones:(1)themostfrequentlyadoptedonewastotesttheinhibitoryeffectincludingantibiosis,mycoparasitismandpathogenlysisofcandidatesonplateandthentesttheefficac
7、yingreenhouseofthemostinhibitorymicroorganisms,followedbyextensiontofieldtrials.Bythisstrategy,anumberofpotentialantagonistswasselectedfromalargenumberofcandidates,andthoseisolateswhichwerelessinhibitoryweredisca
