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《星點(diǎn)設(shè)計-效應(yīng)面法優(yōu)化殼聚糖溫敏凝膠的制備及緩釋性考察》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在教育資源-天天文庫。
1、星點(diǎn)設(shè)計 -效應(yīng)面法優(yōu)化殼聚糖溫敏凝膠的制備及緩釋性考察李曉娟，[基金項目]國家自然科學(xué)基金(81160395)[作者簡介]李曉娟(1987-)，女，碩士研究生，聯(lián)系電話：15999294455，E-Mail:lixiaojuan_1987@126.com[通訊作者]陳文(1967-)，男，教授，從事藥物新劑型研究，聯(lián)系電話：13179930326，E-Mail:chen-wen2000@126.com郭亞可，韓博，陳文(新疆石河子大學(xué)藥學(xué)院，新疆石河子832000)[摘要]目的：通過殼聚糖/β-甘油磷酸鈉（Chitosan/β-Glycerophosphate,CS/β-G
2、P）溫敏凝膠制備工藝的優(yōu)化，為緩釋載體的研究提供基礎(chǔ)。方法：通過考察殼聚糖的脫乙酰度（degreeofdeacetylation,DD）、殼聚糖質(zhì)量、β-GP濃度和pH，選取β-GP濃度和pH為星點(diǎn)設(shè)計考察因素，膠凝時間為考察指標(biāo)，對結(jié)果進(jìn)行多元線性和多項式擬合，效應(yīng)面法優(yōu)化工藝，并以神經(jīng)生長因子（β-nervegrowthfator,β-NGF）為模型藥物，考察CS/β-GP溫敏凝膠的緩釋性，初步探討其釋藥模型。結(jié)果：根據(jù)復(fù)相關(guān)系數(shù)可知，因素與指標(biāo)之間的最佳擬合方程為三次多項式，最優(yōu)工藝：β-GP濃度為511.51mg·mL-1，pH為7.08，膠凝時間為6.23min。且
3、釋藥行為接近Weibull模型。結(jié)論：星點(diǎn)設(shè)計-效應(yīng)面優(yōu)化的CS/β-GP溫敏凝膠工藝，具有膠凝時間短和緩釋性好等優(yōu)點(diǎn)，可滿足植入給藥的要求。[關(guān)鍵詞]星點(diǎn)設(shè)計；效應(yīng)面法；溫敏凝膠；神經(jīng)生長因子；緩釋Studyonpreparationandsustained-releaseofchitosanthermosensitivehydrogelbycentralcompositedesignandresponsesurfacemethodologyLIXiao-juan,GUOYa-ke,HANBo,CHENWen(SchoolofPharmacy,ShiheziUniversi
4、ty,Shihezi,Xinjiang,832000,China)[Abstract]Objective:Thepurposewastooptimizethepreparationprocessofchitosan/β-glycerophosphate(CS/β-GP)thermosensitivehydrogelandtolayafoundationforfurtherresearchofsustained-releasedeliverysystem.Methods:Byexaminingthedegreeofdeacetylation(DD)ofchitosan,theq
5、ualityofchitosan,β-GPconcentrationandpH,thestudyselecttheβ-GPconcentrationandpHasinvestigationfactors,gellingtimeasindex,Theresultsweretofitmultiplelinearorpolynomialandtooptimizetheprocessbyresponsesurfacemethodology.ThestudyonSustained-releaseofCS/β-GPtemperature-sensitivegelasamodeldrugo
6、fnervegrowthfactor(β-NGF),andexploreitsreleasemodel.Results:Theresultsofthemultiplecorrelationcoefficientshowsthatthebest-fitequationbetweenthefactorsandindicatorsforthecubicpolynomial,theoptimum:theconcentrationofβ-GPwas511.51mg·mL-1,pHwas7.08,geltimewas6.23min,andthereleasebehaviorwasclos
7、etotheWeibullmodel.Conclusion:TheoptimizationofCS/β-GPtemperaturesensitivegelbycentralcompositedesign-responsesurfacehastheadvantagesofshortgeltimeandsustained-releasetomeettherequirementsofimplantabledrugdelivery.[KeyWords]centralcompositedesign;respons
