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1、全基因組關(guān)聯(lián)分析饅頭比容與質(zhì)構(gòu)SNP標(biāo)記劉娟1陳廣鳳2田紀(jì)春3吳澎1趙子彤1楊藝1李向陽1唐曉珍1(山東省高校食品加工技術(shù)與質(zhì)量控制重點(diǎn)實(shí)驗(yàn)室;山東農(nóng)業(yè)大學(xué)食品學(xué)院1,泰安271018)(德州學(xué)院生態(tài)與景觀學(xué)院2,德州253023)(山東農(nóng)業(yè)大學(xué)小麥品質(zhì)育種研究室;山東省作物生物學(xué)重點(diǎn)實(shí)驗(yàn)室3,泰安271018)摘要為從分子水平研究控制饅頭比容與質(zhì)構(gòu)性狀的基因位點(diǎn),以205份不同小麥品種為實(shí)驗(yàn)材料,利用分布于小麥全基因組的24355個(gè)單核苷酸多態(tài)性(SNP)標(biāo)記對(duì)饅頭比容與質(zhì)構(gòu)性狀進(jìn)行關(guān)聯(lián)分析。共檢測(cè)到42個(gè)比容性狀顯著關(guān)聯(lián)位點(diǎn),其中8個(gè)極顯著關(guān)聯(lián)位點(diǎn)(P<0.0001)
2、,同時(shí)也是高遺傳貢獻(xiàn)率位點(diǎn)(R2>10%),2個(gè)位點(diǎn)至少在兩個(gè)環(huán)境中穩(wěn)定表達(dá);檢測(cè)到313個(gè)質(zhì)構(gòu)性狀顯著關(guān)聯(lián)位點(diǎn),其中31個(gè)極顯著關(guān)聯(lián)位點(diǎn),46個(gè)高遺傳貢獻(xiàn)率位點(diǎn),11個(gè)位點(diǎn)至少在兩個(gè)環(huán)境中穩(wěn)定表達(dá)。同時(shí),發(fā)掘了5個(gè)質(zhì)構(gòu)性狀主效關(guān)聯(lián)位點(diǎn),如3B染色體上黏聚性關(guān)聯(lián)位點(diǎn)Kukri_c13329_800等。本研究所得到的這些標(biāo)記為在分子水平上研究饅頭品質(zhì)性狀提供了有價(jià)值的參考。關(guān)鍵詞饅頭比容饅頭質(zhì)構(gòu)單核苷酸多態(tài)性標(biāo)記全基因組關(guān)聯(lián)分析顯著關(guān)聯(lián)位點(diǎn)中圖分類號(hào):TS201.1文獻(xiàn)標(biāo)識(shí)碼:AGenome-WideAssociationAnalysisbetweenSNPMarkersa
3、ndSpecificvolumeandTextureRelatedTraitsofSteamedBreadLIUJuan1,CHENGuangfeng2,TIANJichun3,WUPeng1*,ZHAOZitong1,YANGYi1,LIXiangyang1,TANGXiaozhen1(KeyLaboratoryofFoodProcessingTechnologyandQualityControlinShandongProvince;CollegeofFoodScienceandEngineering,ShandongAgriculturalUniversity1,Ta
4、ian271018)(CollegeofEcologyandLandscapeArchitecture,DezhouUniversity2,Dezhou253023)(StateKeyLaboratoryofCropBiology;GroupofQualityWheatBreading,ShandongAgriculturalUniversity3,Taian271018)AbstractInordertostudythegenelocithatcontrolspecificvolumeandtextureofsteamedbread,205diversewheatvar
5、ietieswereusedtoconducttrait-markersassociationusing24355singlenucleotidepolymorphism(SNP)markerscoveredthewholegenomeofwheat.Intheresults,42significantmarkersweredetectedforspecificvolumeofsteamedbreaddistributedacross7chromosomesofwheat,including8highlysignificantmarkers(P<0.0001),aswel
6、lashighgeneticcontributionmarkers(R2>10%),2markersdetectedintwoormoreenvironment.313significantmarkersweredetectedfortexturetraitsofsteamedbreadmappedonto15chromosomesofwheat,including31highlyassociatedmarkers(P<0.0001),46highgeneticcontributionmarkersand11stablemarkers.Atthesametime,5mai
7、nsitesweredetectedfortextureofsteamedbread,suchasKukri_c13329_800onchromosome3B.Theseresultswillfacilitatefurtherresearchesinsensorypropertiesinsteamedbread.基金項(xiàng)目:山東省重點(diǎn)研發(fā)計(jì)劃(公益類)(2017GNC10101)山東農(nóng)業(yè)大學(xué)作物生物學(xué)國家重點(diǎn)實(shí)驗(yàn)室開放課題(2015KF14)收稿日期:2017-10-09作者簡介:劉娟,女,1994年出生,碩士,食品科學(xué)通訊作者