仙靈骨葆對兔膝骨性關(guān)節(jié)炎治療作用的研究

《仙靈骨葆對兔膝骨性關(guān)節(jié)炎治療作用的研究》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。

1、仙靈骨葆對兔膝骨性關(guān)節(jié)炎治療作用的研究作者：程潭，張柳，李震，李強(qiáng)，穆樹林，田發(fā)明，劉永強(qiáng)【摘要】[目的]研究仙靈骨葆(xianlinggubao,XLGB)對兔骨性關(guān)節(jié)炎關(guān)節(jié)軟骨及軟骨下骨的保護(hù)作用。[方法]30只3個(gè)月齡雄性日本大耳白兔，隨機(jī)分為對照組(Sham)、鹽水組(ACLT+NS)和仙靈骨葆組(ACLT+XLGB)，每組各10只。鹽水組和仙靈骨葆組行右膝關(guān)節(jié)前交叉韌帶切斷術(shù)(anteriorcruciateligamenttransaction,ACLT)造成膝骨性關(guān)節(jié)炎模型。術(shù)后仙靈骨葆組125mg/(

2、kg·d)灌胃;鹽水組則給予等劑量生理鹽水。6周后處死動物，取股骨測量骨密度，之后脫鈣制片，行HE染色、Mankin評分及BMP-2、MMP-13免疫組化染色;取右膝脛骨近端包埋，制成硬組織切片，用于骨組織形態(tài)計(jì)量學(xué)測量。[結(jié)果](1)Mankin評分：仙靈骨葆組顯著低于鹽水組。(2)股骨遠(yuǎn)端骨密度及脛骨近端軟骨下骨骨量：仙靈骨葆組均顯著高于鹽水組。(3)免疫組化：仙靈骨葆組MMP-13的表達(dá)顯著低于鹽水組，BMP-2的表達(dá)顯著高于鹽水組。[結(jié)論]仙靈骨葆125mg/(kg·d)灌胃能夠部分阻止骨關(guān)節(jié)炎兔關(guān)節(jié)軟骨的退

3、變，其作用機(jī)制可能與下調(diào)MMP-13的表達(dá)，上調(diào)BMP-2的表達(dá)，同時(shí)增加軟骨下骨骨量，改善其微觀結(jié)構(gòu)有關(guān)?！娟P(guān)鍵詞】仙靈骨葆;骨性關(guān)節(jié)炎;軟骨細(xì)胞;軟骨下骨;BMP-2;MMP-1314　　Abstract:[Objective]ToassesstheprotectiveeffectsofXianlinggubaoonarticularcartilageandsubchondralboneinexperimentalosteoarthritisinducedbyanteriorcruciateligamenttra

4、nsaction(ACLT)inrabbits.[Methods]ThirtyhealthyJapanesewhiterabbitsweredividedrandomlyintothreegroups:shamgroup,ACLT+XLGBgroupandACLT+NSgroup.RabbitsinACLT+XLGBgroupandACLT+NSgroupreceivedanteriorcruciateligamenttransection(ACLT)ontherightknee.Rabbitsinshamgroup

5、receiveduntreatmentascontrol.ACLT+XLGBgroupreceivedadailyadministrationofXLGBatadoseof125mg/kgofbodyweightfor6weeks.Incontrast,ACLT+NSgroupreceivedsalineatthesamedose.Allrabbitsweresacrificedat6weeksafteroperation.Therightfemorawereremoved,measuredforboneminera

6、ldensity(BMD),andthenwereobservedforthemacro-pathologicchangeswithHEstainingandgradedbyMankin'sscale.ExpressionlevelsofBMP-2andMMP-13weredetectedbyimmunostaining.Thesubchondralboneofrighttibialplateauwascutandstainedforthemeasurementofbonehistomorphometry.[Resu

7、lts](1)TheMankin’sscoreinACLT+XLGBgroupwassignificantlylowerthanthatinACLT+NSgroup(P<0.05).(2)TheBMDofdistalfemoraandsubchondralbonemassofproximaltibiainACLT+XLGBgroupwassignificantly14higherthanthatinACLT+NSgroup(P<0.05).(3)InACLT+XLGBgroup:theexpression

8、levelofMMP-13wassignificantlylowerwhileBMP-2wassignificantlyhigherthanthatinACLT+NSgroup(P<0.05).[Conclusion]DailyadministrationofXLGBatadoseof125ug/kg/dfor6weekscanparti