資源描述：
《無色書虱ache基因克隆及其系統(tǒng)進(jìn)化分析》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在教育資源-天天文庫。
1、無色書虱AChE基因克隆及其系統(tǒng)進(jìn)化分析豆威1，唐培安1”，蔣紅波1，王進(jìn)軍1(1．西南大學(xué)植物保護(hù)學(xué)院，昆蟲學(xué)及害蟲控制T程重慶市市級(jí)霞點(diǎn)實(shí)驗(yàn)室，重慶400715；2．南京財(cái)經(jīng)大學(xué)食品科學(xué)與工程學(xué)院，南京210003)摘要：利用RT—PCR結(jié)合RACE技術(shù)克隆獲得了無色書虱尉acel的3’端序列和Ldace2的全長序列(GenBank登錄號(hào)分別為：F1647186和F]647187)。其中Ld∞e2基因全長2111bp，ORF1917bp，編碼638個(gè)氨摹酸組成的前體蛋白，成熟蛋白的分子量為71．9kDa，理論等電點(diǎn)為4．70，序列比對(duì)表
2、明該基因包括AChE家族的所有保守性功能位點(diǎn)；刖acei基因僅獲得了3’端1616bp的序列，編碼500個(gè)氨基酸殘基，序列比對(duì)發(fā)現(xiàn)，除缺少形成一對(duì)二硫鍵的半胱氨酸外，從acel包含了AChE家族的所有保守性功能位點(diǎn)。結(jié)合其它物種AChE構(gòu)建的分子進(jìn)化樹可以看出，AChE分為4個(gè)類群：昆蟲I型AChE類群、脊椎動(dòng)物AChE類群、昆蟲Ⅱ型AChE類群和線蟲AChE類群，其中無色書虱尉acel和蹦ace2分別屬于昆蟲I型AChE類群和昆蟲Ⅱ型AChE類群，且與嗜卷書虱和嗜蟲書虱的AChE具有最近的進(jìn)化關(guān)系。關(guān)鍵詞：無色書虱；乙酰膽堿酯酶；基因克隆
3、；系統(tǒng)發(fā)育中圖分類號(hào)：Q968文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：1674-0858(20LO)04-0460一09GenecloningofAChEandphylogeneticanalysisinLiposcelisdecslorDOUWeil，TANGPei—An2，JIANGHong—B01，WANGJin—Jun¨(1．KeyLaboratoryofEntomologyandPestControlEngineering，CollegeofPlantProtection，SouthwestUniversity，Chongqing400715，C
4、hina；2．CollegeofFoodScienceandEngineering，NanjingUniversityofFinanceandEconomics，Nan-jing210003，China)Abstract：ThefulllengthofLdace2andpartialfragmentof甜acel(GenBankaccessionnumber：FJ647187andFJ647186)wereclonedfromLiposcelisdecolor，respectively．ThecompleteeDNAofLdace2cons
5、istsof21llbp塒thanORFofl917bp。encodingaproteinof638aminoacidsresidues．Thematureproteinhasacalculatedmolecui甜weightof71．9kDawithanisoelectricpointof4．7．AllthetypicalresiduesofAChEfamilywerefoundinthededucedaminoacidsequenceofLdace2bysequencealignment．ThefragmentofLdacelconta
6、insl6l6bpencoding500aminoacidresidues．TheaminoacidsequencehasahighidentitytoAChEIfrom厶bostrychophilaandLentomophila．ItincludesallthetypicalresiduesofAChEfamilyexceptoneconservedcysteinethatcomposesdisulfidebridge．nephilo-genetictreeof50AChEaminoacidsequencesfrom31specieswe
7、reconstructedbyMEGA4．1．There—suitsshowedthatallAChEgenesweredividedintofourdistinctmaingroups：InsectTypeIAChEGene，VertebmteAChEGene，InsectTypeIIAChEGeneandNematodeAChEGene．LdacelandLdace2fromL&colorwereclassifiedintoInsectTypeIAChEGeneandInsectTypeIIAChEGene，respectively．K
8、eywords：Liposcelisdecolor；acetylcholinesterases；geneclone；phylogeny乙酰膽堿酯酶(Acetylcholinest
