竹蓀提取液對(duì)砷中毒大鼠血細(xì)胞參數(shù)的影響.doc

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1、竹蓀提取液對(duì)砷中毒大鼠血細(xì)胞參數(shù)的影響作者：羅鵬，張愛華，吳應(yīng)寬，汪希蘭，潘雪莉【摘要】目的：分析竹蓀提取液對(duì)砷中毒大鼠血細(xì)胞15項(xiàng)參數(shù)的影響，為防治砷中毒及竹蓀資源的開發(fā)利用提供依據(jù)。方法：As2O3經(jīng)口染毒，建立砷中毒動(dòng)物模型，竹蓀提取液灌胃14d，采用F820半自動(dòng)血細(xì)胞分析儀測(cè)定實(shí)驗(yàn)動(dòng)物血細(xì)胞參數(shù)。結(jié)果：（1）與正常對(duì)照組比較，砷中毒模型組紅細(xì)胞計(jì)數(shù)（RBC）、紅細(xì)胞壓積（HCT）、淋巴細(xì)胞絕對(duì)值（WSCC）、血小板（PLT）顯著降低，P<0.05或P<0.01；紅細(xì)胞血紅蛋白含量（MCH）、平均紅細(xì)胞血紅蛋白濃度（MCHC）顯著升高，P

2、<0.05或P<0.01；（2）與砷中毒模型組比較，竹蓀治療組RBC、血紅蛋白（HGB）、HCT呈現(xiàn)升高趨勢(shì)，MCH呈降低趨勢(shì)，MCHC隨竹蓀劑量升高而降低，高劑量組差異具有顯著性（P<0.05），平均紅細(xì)胞體積（MCV）、紅細(xì)胞體積寬度（RDWCV）呈升高趨勢(shì)且高于正常對(duì)照組；白細(xì)胞（WBC）、WSCC、中性粒細(xì)胞絕對(duì)值（WLCC）呈上升趨勢(shì)；淋巴細(xì)胞百分?jǐn)?shù)（WSCR）在竹蓀低劑量組顯著性降低（P<0.05），而后隨劑量增加呈上升趨勢(shì)；中性粒細(xì)胞百分?jǐn)?shù)（WLCR）在竹蓀低劑量組顯著性升高（P<0.05），而后隨劑量增加呈

3、下降趨勢(shì)；PLT持續(xù)降低，血小板分布寬度（PDW）、平均血小板體積（MPV）呈上升趨勢(shì)，但差異無統(tǒng)計(jì)學(xué)意義。結(jié)論：AS2O3對(duì)實(shí)驗(yàn)大鼠血液系統(tǒng)有一定的損害作用，竹蓀提取液對(duì)砷所致的大鼠血液系統(tǒng)紅細(xì)胞、白細(xì)胞、血小板等指標(biāo)具有一定的保護(hù)作用?！娟P(guān)鍵詞】砷中毒；紅細(xì)胞；白細(xì)胞；血小板；竹蓀提取液；大鼠，Wister　　［Abstract］Objective:Toanalyze15bloodparmetersinarseniasisratsthatchangedundertheinfluenceofextractfromDictyophoraduplicate(EXD

4、),soastoprovidereferencesforfurtherstudy.Methods:ArsenicpoisoninganimalmodelwasestablishedbygivenAS2O3toratsorally.EXTwasthanusedtotreattheseratsorallyfor14days,andF820semiautomaticbloodcorpuscleanalyzerwasadoptedtoanalyzethebloodparametersofexperimentalanimals.Results:(1)Comparedw

5、iththoseofnormalcontrolgroup(groupC),RBC,HCT,WSCC,andPLTwerelowerandMCH,andMCHCwerehighersignificantlyinarsenicpoisoninggroup(groupAP)(P﹤0.05orP﹤0.01);(2)InEXDtreatedgroups(highandlowdoses),RBC,HGB,HCT,MCV,andRDWCVwerehigherthanthoseofgroupAP,andMCV,andRDWCVwerehigherthanthoseofgr

6、oupC.Whendoseincreased,MCHCdescendedsignificantlyinhighdosegroup(P﹤0.05).WBC,WSCC,andWLCCincreasedmorealongwithdoseraise.WSCRdescendedsignificantlyinthelowdosegroup(P﹤0.05),butincreasedmorewhendoseincreased.WLCRhadoppositedisplaytoWSCR.PLTcontinuouslyreducedalongwithdoseraise.PD

7、WandMPVshowedarise4trend,butitwasnotstatisticallysignificant.Conclusions:AS2O3hasharmfuleffectonbloodsystemofexperimentalratstosomeextent,andEXDhasshelteringeffectonbloodsystem,andcancausechangesoferythrocye,leucocyte,andplateletindexes,butthemechanismsneedtobestudiedfurther.［Keyword

8、s］arsenicpoi