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《rho／rock信號(hào)通路在腹膜透析大鼠腹膜纖維化中的作用及法舒地爾的干預(yù)效應(yīng)》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、Rho/ROCK信號(hào)通路在腹膜透析大鼠腹膜纖維化中的作用及法舒地爾的干預(yù)效應(yīng)孟緒初1甘平2(通訊作者)(1貴州醫(yī)科大學(xué)研究生學(xué)院貴州貴陽(yáng)550004)(2貴州醫(yī)科大學(xué)附屬烏當(dāng)醫(yī)院腎內(nèi)科貴州貴陽(yáng)550018)【摘要】目的：探討Rho/ROCK信號(hào)通路在腹膜透析大鼠腹膜纖維化屮的可能機(jī)制。方法:將30只雄性SD大鼠隨機(jī)分為四組：對(duì)照組，每日腹腔注射生理鹽水25ml;模型組，每口腹腔注射4.25%的腹透液25ml;Fasudil組，每口腹腔注射含法舒地爾(Fasudil)濃度為10mg/Kg?d的4.25%腹透液2
2、5ml。實(shí)驗(yàn)第28天，取壁層腹膜組織行HE染色，并測(cè)定壁層腹膜ROCK-1的表達(dá)情況及SOD、MDA含量。結(jié)果：與模型組比較，F(xiàn)asudil組不同程度腹膜厚度減少、膠原纖維沉積減輕，腹膜組織ROCK-1表達(dá)減少(P<0.05)，MDA含量降低(P<0.05),SOD活性增加(P<0.05)。結(jié)論：腹膜透析液激活丫腹膜透析人鼠腹膜Rho/ROCK信號(hào)通路，促進(jìn)腹膜組織氧化應(yīng)激損傷，導(dǎo)致丫腹膜纖維化的發(fā)生，ROCK抑制劑法舒地爾能通過(guò)抑制此通路減少腹膜的氧化應(yīng)激反應(yīng)，進(jìn)而延緩腹膜纖維化進(jìn)展。【關(guān)
3、鍵詞】腹膜透析；腹膜纖維化；氧化應(yīng)激；Rho/ROCK信號(hào)通路【屮圖分類號(hào)】R459.5【文獻(xiàn)標(biāo)識(shí)碼】A【文章編號(hào)】2095-1752(2016)08-0059-03Rho/ROCKsignalingpathwayinpdratperitonealfibrosis,theeffectoftheinterventioneffectofshu,andthemethodMengXuchu1GanPing2.1GuizhouGuiZhouGuiYangmedicalUniversitygraduateschoolof
4、550004,China.2WuwhenHospitalaffiliatedtomedicalUniversityrenalmedicineinguizhouGuiZhouGuiYang550018,China【Abstract】ObjectiveTostudytheRho/ROCKsignalingpathwayinthepossiblemechanismofpdratperitonealfibrosis.Methods30maleSDratswererandomlydividedintofourgroup
5、s:controlgroup,thedailyintraperitonealinjectionofsaline25ml;Modelgroup,thedailyintraperitonealinjectionof4.25%oftheliquidtodrain25ml;ByintraperitonealinjectionofFasudilgroup,dailywithshutofarmar(Fasudil)concentrationof10mg/Kg,4.25%ofthedtodrainfluid25ml.Exp
6、eriment28days,parietallayerandorganizationallineHEstaining,andthedeterminationofparietallayerandtheexpressionofROCK-1andSOD,MDAcontent.ResultsComparedwithmodelgroup,differentFasudilgroupperitonealthicknessreduce,collagenfibersdepositionreduction,reducedperi
7、tonealorganizationROCK-1expression(P<0.05),lowerMDAcontentincreased(P<0.05),SODactivity(P<0.05).ConclusionPeritonealdialysateactivatesthepdratperitonealRho/ROCKsignalingpathway,promotetheperitonealtissuesoxidativestressdamage,causedtheincidenceofpe
8、ritonealfibrosis,shutofarmarROCKinhibitorscaninhibitthepathwaytoreduceperitonealoxidativestressresponse,anddelaytheprogressofperitonealfibrosis.【Keywords］Peritonealdialysis;Peritonealfibrosis;Oxidative
