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ID:28470625
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時間:2018-12-10
《姜黃素結合有氧運動對中老年大鼠骨骼肌aktmtor信號通路的影響》由會員上傳分享,免費在線閱讀,更多相關內容在應用文檔-天天文庫。
1、ABSTRACTObjective:BydetectingtheratsoleusmuscleIGF一1,changePi3k/Akt/mTORproteinexpressionofitscontenttocellapoptosis,explorecurcumin,constantloadexercise,incrementalExerciseonPi3k/Akt/mTORsignalingpathway.Andcomparingcurcumin,constantloadexercise,incrementalloadexercise,combinedw
2、ithconstantloadexercisecurcumin,curcumincombinedratskeletalmuscleagingeffectofexerciseonincreasingloaddelay.Methods:Thesamebatchof18healthymaleSDratswere60monthsofage(555.61±57.449),SPF(SpecificpathogenFree,SPF)levelofanimals,housedseparately.Adaptivefeedingforthreedays,wererando
3、mlydividedintosixgroups,wererandomlydividedintocontrolgroup(Controlgroup,BC,n=lO),curcumin(Curcumingroup,QC,n=lO),constantloadexercisegroup(Constantloadexercisegroup,CE,n=10),increasingloadexercisegroup(incrementalExercisegroup,IE,n=lO),aconstantloadexercise+curcumingroup(constan
4、tloadexercisecurcumingroup,CC,n=10),incrementalExercise+curcumingroup(IncrementalExercise+curcumingroup,IC,n=lO).2pointseachtocages,fivepercage.Exercisegroupratsusingtreadmillexercise,theestablishmentofaconstantloadand5、gtoTable卜2.Slopeof0(equivalenttoExerciseIntensity70%V02max),fivedaysaweekfortraining,twodaysofrest,trainingatotalof8weeks.AftertheendIII萬方數據ofthelast20hoursoftrainingtotakeblood,soleusmusclewasmeasuredIGF一1contentusingE1issamethod,usingWesternBlottingDetectionAkt,Pi3k,mTORprotein6、expression,andthesituationofskeletalmusclecellapoptosisbyTUNELassay.Results:(1)Muscleapoptosisinratsoleussmallestindexisconstantloadexercisegroup,followedbythecurcumingroup,一.一●●1一apoptotiCindex1Sthelargestgrouplncreaslngload.Comparedwithcurcumingroupandconstantloadgroup,therewas7、asignificantdifferenceP<0.01:comparedwithcurcuminincreasingloadwassignificantdifferenceP<0.05.(2)comparedwiththegroupandcurcumingroupconstantload,1。ncreasinggloadoadinserumIGF—l—contentwasS111candifference(P8、tdifference(P<0.05)musclecontentofIGF-Ii
5、gtoTable卜2.Slopeof0(equivalenttoExerciseIntensity70%V02max),fivedaysaweekfortraining,twodaysofrest,trainingatotalof8weeks.AftertheendIII萬方數據ofthelast20hoursoftrainingtotakeblood,soleusmusclewasmeasuredIGF一1contentusingE1issamethod,usingWesternBlottingDetectionAkt,Pi3k,mTORprotein
6、expression,andthesituationofskeletalmusclecellapoptosisbyTUNELassay.Results:(1)Muscleapoptosisinratsoleussmallestindexisconstantloadexercisegroup,followedbythecurcumingroup,一.一●●1一apoptotiCindex1Sthelargestgrouplncreaslngload.Comparedwithcurcumingroupandconstantloadgroup,therewas
7、asignificantdifferenceP<0.01:comparedwithcurcuminincreasingloadwassignificantdifferenceP<0.05.(2)comparedwiththegroupandcurcumingroupconstantload,1。ncreasinggloadoadinserumIGF—l—contentwasS111candifference(P8、tdifference(P<0.05)musclecontentofIGF-Ii
8、tdifference(P<0.05)musclecontentofIGF-Ii
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