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《玉米花粉誘導(dǎo)小黑麥單倍體技術(shù)建立與單倍體誘導(dǎo)率遺傳規(guī)律分析》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、關(guān)鍵詞：小黑麥；玉米花粉；單倍體；遺傳規(guī)律論文類型：B（應(yīng)用研究）IIAbstractHaploidbreedingisapowerfultooltoenhancebreedingprocessandimprovebreedingefficiency.Andantherorpollencultureismainlyappliedintraditionalhaploidbreeding.However,thecomplexprocessandhigherfrequencyofcolonelvariationmakeantherorpollencu
2、lturequitedifficultinlargescalesapplication.Itissimpleandfeasibletoobtainwheathaploidinducedbymaizepollen.Thepresentstudyisaimedtoestablishthetechnologyoftriticalehaploidinducedbymaizepollen,andunderstandgeneticofhaploidinductionrate.Thisstudywillprovidesometheoreticalbasis’
3、sforanefficienttriticalebreedingtechnology.Somewinterandspringtriticalegenotypeswereinducedbyfourmethods,A:maizepollenandchemicals(inducedbymaizepollenandsprayedwithDMSO40ml/L,COL4g/L,Inositol100mg/L,IAA20mg/Land2,4-D20mg/L）;B:chemicals（DMSO40ml/L,COL4g/L,Inositol100mg/L,IAA
4、20mg/Land2,4-D20mg/L）;C:maizepollenandhormone(inducedbymaizepollen,thensprayedwith100mg/L2,4-Dand30ml/LDMSO);D:hormoneonly(100mg/L2,4-Dand30ml/LDMSO).Theeffectsofhaploidembryosrescuingmedium,maizegenotypeandtriticalegenotypeonhaploidembryoinductionwerealsostudiedtoestablisht
5、hetechnologyofinducingtriticalehaploidbymaizepollen.Throughthecomplete6×6diallelcrossingbetween6triticalevarietieswithdifferenthaploidembryofrequency,thecombinationability,geneticeffect,heterosisofhaploidembryofrequenciesofparent’sheritabilityandgeneticcorrelationofF1offspri
6、ngwereanalyzed.Themainresultswereasfollows:1.Inductionbymaizepollenandhormoneisthebestmeansamongthefourmethodstoinducetriticalehaploidembryofor6.65%haploidembryos,whichishigherthantheother3methodsinthisexperiment.2.Haploidembryocouldbeinducedbymaizepollenandchemicalsinmosttr
7、iticalegenotypes.However,thetriticalehaploidinductionratesvariedamongdifferentgenotypes.Thetriticalegenotype07H261-62/NTH2146-2isthebestonewithhigherinductionratehaploidembryo.Theinductionratesofhaploidembryodifferentiatesignificantlyamongdifferenttypesofmaizetoo,andwaxycorn
8、isthebestoneamongthethreemaizetypestestedintheexperiment.3.Thereisnosignifi
