腎移植術(shù)后hcmv激活感染和淋巴細(xì)胞亞群及腎功能相關(guān)性

《腎移植術(shù)后hcmv激活感染和淋巴細(xì)胞亞群及腎功能相關(guān)性》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。

1、AbstractObjective：ToinvestigatecorrelationbetweenHCMVactivatedinfectionwithTlymphocytesubsets、HLAandrenalfunctionchangesinrenaltransplantation．Becauseofpreoperativedialysisandpostoperativeimmunosuppressant，renaltransplantpatientsalwayshaveHCMVprimaryinfectionandlatentinfectionactiv

2、atedopportunity．Methods：45patientsofrenaltransplantwereselected．Preoperativeandpostoperativebloodspecimensweretrackingcollected．HCMVactivatedinfectionwasdetectedbyRT—PCR，whilelymphocytesubsetsusedflowcytometrytest．Results：(1)HCMVactivatedinfectionratewas11．1％beforerenaltransplantat

3、ionand57．8％after(2)AfterrenaltransplantationtheHCMVactivatedinfectiongrouprisedCD4+，CD8+、CD4+／CD8+reduceeveninverted，comparedwithHCMVnoactivatedinfectiongroup，withstatisticaldifference(P

4、％)，HCMVnoactivatedinfectiongroupoccurredin1case(5-3％)，thedifferencehasstatisticalsignificancefP2withninepeople．HCMVIE2positiverate

5、wasstatisticallydifferencebetweentwogroups(P<0．05)，nostatisticaldifferenceinHCMVIE2positivehappentimefP>O．05)．Conclusion：TlymphocytesubsetschangesandrenalfunctionrelatedHCMVactivatedinfectioninrenaltransplantationclosely，especiallyCD4+／CD8+．ExcessiveconcentrationofHLAmismatchingwer

6、eassociatedwithHCMVactivatedinfection．Theywerecorrelatedandmutualcausal，whileclinicaldiagnosisandtreatmentandprognosishadcertainsignificancefollowfurthermechanismresearch．Masterstudent：Peng-liu(Pathogenicmicroorganism)Directedbyprof：Bin-wangKeywords：HumancytomegalovirusIE2；Renaltra

7、nsplantation；Lymphocytessubsets；HLA目錄引言?????????????????????????????????????????????·1第1章材料與方法???????????????????????????31．1標(biāo)本來(lái)源??????????????????????????????··31．2儀器與試劑?????????????????????????????··31．3常用試劑配制????????????????????????????··41．4方法???????????????????????????????????

8、????????·41．5實(shí)驗(yàn)分組與HCMV激活感染