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《松材線蟲病發(fā)病植株細(xì)菌的分離鑒定與綠僵菌mf2菌株殺線測(cè)定》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、松材線蟲病發(fā)病植株細(xì)菌的分離鑒定及綠僵菌Mf2菌株殺線測(cè)定ABSTRACTPinewiltdiseasewhichisadevastatingdiseaseonpinetreesisanimportantinternationalrecognizedquarantinedisease．Atpresentpeoplehasdonealotofresearchonitspathogenandpathogenicmechanism，butthereisn’tallexactconclusionuntilnow．Thisarticleproposed'tolookforanewbreakthr
2、oughinpathogenesisbyanalyzingofthechangingofmicrobiota證thediseasedpinetree．Inadditlon．thenematicidalactivityofMetarhiziumanisopliaeMt"2strainWastested,hymgthefoundationforusingMetarhiziumanisopliaeasbiologicalpesticidetoconlrolBursaphelenchusxylophilus．Inthisresearch，bacteriaformhealthyanddiseas
3、ed15yearsoldPinusmassonianawereis01ated．Theresultshowsthat：1、TheewerealargenumberofbacteriaappearedintheLBflatmediumwiththediseasedplantwoodonedaylater，however，thehealthonehadnothing．2、purifiedthebacteriaand12strainswereisolated．ExtractedbacterialgenomicDNA,identfliedthebacteriausingthemethodofm
4、olecularappraisalandgottheresultsthatPectobacteriumcypripedi,iEnt甜obaaerasburiae，Enterobactersp．．MetarhiziumanisopliaeisoneofthemostappliedentopathogeneticfungkInthisstudy,onemediumWaschooseaftercomparisonamongfourcommonmediaPDA、PPDA、SDAYandSMAYwhichismostsuitableforvegetativegrowthandsporeprodu
5、ctionofanisopliaestrainsMf2．WetestedtheinsecticidaleffectivenessofManisopliaeto。pinewoodnematode．TheresultsshowedthattherateofgrowthofⅣ【f2ishighestandthediameterofcolonyafter14daysofMf2islargestinthePPDAmedium．Thediameterofcolonyafter14daysis76．00mln．ConidiaproductionofMf2ishighestinthePPDAmediu
6、mwhichis2．5timesofthesecondhighestone．M．a(chǎn)nisopliaehavehighnematicideactivitytopinewoodnematode．Themortalityafter36houris98．52％．Pinewoodnematodesarecompletelydissipatedafter5days．M．a(chǎn)n西opliaehasagoodapplicationpotentialtocontrolpineWiltdisease．··Keywords：pinewiltdisease，bacteria，Metarh&iumanisopli
7、ae，BursaphelenchusxylophilusAcrBisbpdDNADNTPEDTAghH202kbLBNAODPCRPDASDSTEMEDTriS縮略詞acn／lamideN—N’-MethylenebisacrylamidebasepairsDaydeoxyr．bonucleicaciddeoxyribonucleotidetriphosphateethylenediaminetetraaceticacidGramHourhyd
