資源描述：
《est-ssr標(biāo)記開(kāi)發(fā)與蓮種質(zhì)資源遺傳多樣性分析》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、ShanghaiNormalUniversityMasterofPhilosophyAbstractThesistopic:DevelopmentofSSRMarkersandTheirUtilizationinGeneticDiversityAnalysisofNelumboProfessionaldisciplines:BotanyDegreeapplicant:YuxianXuInstructor:Prof.DaikeTianProf.JianguoCaoAbstractLotus（NelumboAdands）isoneoftheear
2、liestorigintaxainangiosperms.Ithasalonghistoryofcultivationanddiversecultivarswithedible,medicinal,ornamentalvalues.However,classificationandgeneticrelationshiponmanycultivarsremainunclear.Theapproachonlybasedonthetraditionalmorphologyisdifficulttoaccuratelyidentifyacultiva
3、randitsrelationshipwithothers.Inaddition,somewildlotusresourcesarebecomingendangeredTherefore,scientificevaluationandprotectivemeasuresshouldbeimmediatelytakenonlotusgermplasm.Althoughsomemolecularmarkershavebeenrecentlyusedtoinvestigategeneticdiversityandpopulationrelation
4、shipinNelumbo,theapplicationofEST-SSRmarkersandtagnumberareyetlimited.SSRmarkersweredevelopedfromflower-budtranscriptomesequencesofNelumbonuciferainthisstudy,and80polymorphicEST–SSRmarkerstoevaluatethegeneticdiversityofthe139lotusaccessions.Themainresultsareasfollows:1.Inth
5、isstudy,68,593unigeneswereassembledfromamixedflower-budcDNApool,whichwasderivedfromthreeaccessionsofN.nuciferatodevelopSSRmarkers.Atotalof6086SSRlociwereidentifiedanddistributedin5482unigenes,accountingfor8.0%ofthetranscriptomesequences,withanaverageofoneSSRlocusper5.7kbDNA
6、.ThedistributionofSSRlocifrequencywas8.9%.Di-andtri-nucleotiderepeatmotifswerethemajorabundanttypesamong5226SSRloci（removingmononucleotiderepeats）identifiedwithafrequencyof65.2%and31.7%,respectively.Moreover,theAG/CT(57.9%)andGAA/AGA/AAG(13.3%)motifswerepredominantforthedi-
7、andtri-nucleotiderepeats,respectively.Atotalof575primerpairs(fragmentsizerangingfrom200bpto220bp)wererandomlysynthesizedfrom3,059SSRloci,ofwhich514(89.4%)yieldedPCRamplificationIII萬(wàn)方數(shù)據(jù)AbstractShanghaiNormalUniversityMasterofPhilosophyproductsand217(42.2%)polymorphicSSRmarke
8、rs.2.The80polymorphicEST–SSRmarkerswerescreenedfromourstudy,andthenappliedtoamplif
