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1、上海海洋大學(xué)碩士畢業(yè)論文StudiesOnEarlySexDifferentiationAndSex-associatedSNPsScreeningOfTurbots（ScophthalmusMaximusL.）ABSTRACTTheturbot(ScophthalmusmaximusL.)isaflatfishofgreatcommercialvalue,whereastrongsexualdimorphismexistsforgrowthrate.Inthisstudy,byusinghaematoxylin-eosi
2、nstainingofhistologysectionofthegonadsandmacroscopicexaminationfistly,phenotypicsexofjuvenileindividualsfrom3to5monthsagewereidentified.Thensexratioof4022individualswithin37familiesat4monthswereanalyzedbothatfamilyandpopulationlevel.Meanwhile,2254individualsfrom20
3、familiesamong37familieswererandomlyselectedforanalysisofsexualdimorphisminbodyweight.CandidateSNPlocicomefromlarge-scalehigh-throughputsexspecialtranscriptome454sequencingsystemofturbot(ScophthalmusmaximusL.).AccordingtothecandidateSNPloci,primersweredesignedtotes
4、tandverify.AndbyuseofMassArray(MALDI-TOFMSArray),ahigh-throughputgenotypingtechnologyforSNPs,398sexassociatedcandidateSNPswerescreenedandverifiedwith20malesandfemalesat8monthsage.Thentheother35sex-associatedcandidateSNPsof6contigswereverifiedandconductedhaplotypes
5、analysis.Themainresultsareasfollows:1.Bydissectingindividualsat4monthsagedirectly,significantshapedifferenceofgonadsbetweenmaleandfemaleswereobserved.Females’wereshortrod-likewhilemalesaslenderline-like.Then,sexofmost4monthsageindividualscouldbeidentifiedbymacrosc
6、opicexamination.Fewindividualsthatshapeofgonadsshowednosignificantdifferencebetweensexes,stillshouldbeidentifiedbyuseofhaematoxylin-eosinstainingofhistologysectionoftheirgonads.Andashapedifferenceofgonadswasobservedbetween4and6monthsageindividuals,whichsuggestedth
7、atshapeofturbotgonadswascontinuouslychangingduringearlystage.2.Phenotypicsexof4022individualscomposedof37familiesat4monthsagewereidentifiedandcounted.Thestatisticalanalysisshowedthatthenumberoffemaleandmalewere2426and1596,respectively,whichreflectedasignificantdif
8、ference(P<0.01)III萬方數(shù)據(jù)上海海洋大學(xué)碩士畢業(yè)論文insexratiotothehypothesisofabalancedsexratio(50%males/50%females).Andatfamilylevel,therewere19familiesamong37familiese
