2、犯缺損。(3)脛骨組織學(xué)：組織學(xué)檢測(cè)發(fā)現(xiàn)所有的模型組大鼠脛骨髓腔內(nèi)充滿了腫瘤細(xì)胞，骨結(jié)構(gòu)改變明顯，而N組、SN組、SN200組未見骨結(jié)構(gòu)改變。(4)免疫熒光染色：SN卟SG。組中DHSC和DRG內(nèi)未見明顯CCL2表達(dá)，BNⅡ組中DHSC和DRG內(nèi)趨化因子CCL2大量表達(dá)，應(yīng)用加巴噴丁后BGⅡ組DHSC和DRG內(nèi)CCL2的表達(dá)量較BNⅡ組減少(P．<0．05)，但是不能完全抑制。結(jié)論加巴噴丁可以減輕脛骨癌痛大鼠的機(jī)械痛敏，其脊髓機(jī)制可能與抑制DHSC和DR(3中趨化因子CCL2的分泌有關(guān)。關(guān)鍵詞骨癌痛；加巴噴??；背根神經(jīng)節(jié)；脊髓背角；趨化因子CCL2徐州醫(yī)學(xué)
3、院碩士學(xué)位論文EffectofgabapentinonmechanicalaHodyniaandthespinalmechanisminaratmodeloftibialbonecancerpainAbstractobjecfiveToobservetheeffectofgabapentinonmechanicalallodyniainaratmodeloftibialbonecancerpain，andinvestigateifgabap刪nCaninhibitthesecretionofchemokineCCL2inDHSCandDRGMethodsP
4、artITotalof42femaleSprague—Dawleyratswererandomlydividedinto7groups(n=6each)：naivegroup(groupN)，shamoperation+NS(groupSN)，shamoperation+GBP200mg／kg(groupSG200)，BCP+NS(groupBN)，BCP+GBP50mg／kg(groupBG50)，BCP+GBP100mg／kg(groupBGl00)，andBCP+GBP200mg／kg(groupBG200)．TheratsingroupN，SNan
5、dBNreceived5mlnormalsalineandtheratsingroupSG200，BG50，BGl00andBG200received200，50，100and200mg／kgdoseofGBPviafeedingfromday7to13afteroperationperday,respectively．Mechanicalwithdrawalthreshold(MWT)oftherightpawandbehavioralassaysforambulatorypainweremeasul'cdjustbeforeoperationandon
6、days1,3，5，7，8，10，12and14afteroperation．Atlastthetibiawasstainedwithhematoxylinandeosin(HE)toverifycancercellinfiltrationandbonedestruction．Part1ITotalof32femaleSprague-Dawleyratswererandomlydividedinto4groups(n=8each)：shamoperation+NS(groupSNII)，shamoperation+GBP(groupSG)O，BCP+NS(
7、groupBNn)，BCP+GBP(groupBGⅡ)．Themechanicalwithdrawlthreshold(MWT)WaSmeasuredbeforeoperationand1,3，5，7daysafteroperation．Eachgroupaccepteddifferentdrugtherapiesafterlhon7dayswhentheN【W(wǎng)Twasmeasured．GroupSNIIandBNnweregavagedwith4ml／kgNS，andGroupSGnandBG]1weregavagedwith200mg／kgGBPdis
8、solvedin4ml／kgNS．MWTWastestedon2,
