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1、第39卷第2期解剖學報Vol139,No122008年4月ACTAANATOMICASINICAApr.2008·187·中藥何首烏飲抗大鼠卵巢組織衰老的機理1,23413122張娜李亞麗牛嗣云高福祿陳雪郭麗娜趙素英(11河北醫(yī)科大學組織學胚胎學教研室,石家莊050017;21河北醫(yī)科大學第四醫(yī)院生殖醫(yī)學科,石家莊050011;31河北省人民醫(yī)院三優(yōu)中心,石家莊050071;41承德醫(yī)學院基礎醫(yī)學研究所,承德067000)[摘要]目的探討何首烏飲抗大鼠卵巢組織衰老的機理。方法36只SD大鼠隨機分為3
2、組:正常組、模型組、預防組。采用D2半乳糖連續(xù)腹腔注射法制造雌性大鼠亞急性衰老模型,預防組在造模的同時以中藥方劑何ARF首烏飲灌胃。應用逆轉(zhuǎn)錄聚合酶連反應(RT2PCR)方法檢測各組大鼠卵巢組織p53、p19、Rb及p16基因的表達,用Westernblotting方法檢測各組大鼠卵巢組織Rb及p16蛋白的表達情況。結(jié)果何首烏飲明顯抑制了衰老大鼠ARF卵巢組織中p53、p19、Rb以及p16基因表達的上調(diào)趨勢(P<0105),且明顯降低了衰老大鼠卵巢組織中Rb及p16ARF蛋白的表達(P<0105)
3、。結(jié)論何首烏飲抑制衰老途徑中關(guān)鍵的調(diào)節(jié)因子p53、p19、Rb及p16的表達,從而起到抗大鼠性腺衰老的作用。[關(guān)鍵詞]何首烏飲;衰老;p53;Rb;逆轉(zhuǎn)錄聚合酶連反應;免疫印跡法;大鼠[中圖分類號]Q9523,R21217[文獻標志碼]A[文章編號]052921356(2008)022187STUDYONANTI2AGINGINTHEOVARYOFTHEAGINGMODELRATBYTRADITIONALCHINESEMEDICINEHESHOUWUYINZHANGNa1,2,LIYa2li3,NI
4、USi2yun4,GAOFu2lu13,CHENXue1,GUOLi2na2,ZHAOSu2ying2(11DepartmentofHistologyandEmbryologyHebeiMedicalUniversity,Shijiazhuang050017,China;21DepartmentofReproductiveMedicine,theForthHospitalofHebeiMedicalUniversity,Shijiazhuang050011,China;31CenterofHealt
5、hyBirth,ExcellentGrowthandEducationthePeopleHospitalofHebeiProvince,Shijiazhuan050071,China;41InstituteofBasicMedicalSciences,theHospitalofChengdeMedicalCollege,Chengde067000,China)[Abstract]ObjectiveTofindthemechanismoftraditionalChinesemedicineHeshou
6、wuyin(HSWY)inanti2aginginovaryoftheagingmodelrats,soastoprovidetheoreticalandexperimentalevidencetoanti2aginginfemalesexualgland.MethodsThirty2sixratsweredividedintothreegroupsatrandom:normalgroup,agingmodelgroup,preventaginggroup.Ratstheinpreventaging
7、groupweresubdividedintofourgroups:HSWYlowdosagegroup,HSWYmediumdosagegroup,HSWYhighdosagegroupandHeshouwan(HSWW)group.Thesub2acutelyagingmodelratswereinducedbyD2galactose,andratsinthepreventaginggroupwerereceivedintragastricadministrationofHSWYandHSWWw
8、ithD2galactoseintraperionealinjection.TheARFgeneexpressionsofp53,p19,Rbandp16weredetectedintheovariesofeachgroupwithreversetranscriptionpolymerasechainreaction,andtheproteinexpressionsofRbandp16intheovarieswithWesternblotting.ResultsThe