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1、分類號:R758.6密級:不保密UDC:610學(xué)校代碼:11065碩士學(xué)位論文凋亡相關(guān)蛋白在扁平苔蘚中的表達及意義葛晶指導(dǎo)教師王桂芝教授王君副教授學(xué)科專業(yè)名稱皮膚病與性病學(xué)論文答辯日期2015年6月6日摘要凋亡相關(guān)蛋白在扁平苔蘚中的表達及意義摘要目的探討扁平苔蘚皮損中核因子-κB(nuclearfactorkappaB,NF-κB)和凋亡誘導(dǎo)因子(apoptosis-inducingfactor,AIF)的表達及意義。方法采用免疫組化法和脫氧核糖核苷酸末端轉(zhuǎn)移酶介導(dǎo)的原位缺口末端標記技術(shù)(Term
2、inaldeoxynucleotidyltransferase-mediateddUTPNikeEndLabeling,TUNEL)檢測25例扁平苔蘚患者皮損和25例健康皮膚組織中AIF和NF-κB的積分光密度值(integratedopticaldensity,IOD)和角質(zhì)形成細胞的凋亡指數(shù)(apoptosisindex,AI)。結(jié)果扁平苔蘚組NF-κB和AIF的積分光密度值均高于健康對照組(t=3.14和4.09,P<0.01),扁平苔蘚中角質(zhì)形成細胞的凋亡指數(shù)顯著高于健康對照組,兩組比較差
3、異具有統(tǒng)計學(xué)意義(t=4.97,P<0.01)。NF-κB的陽性表達強度與自身角質(zhì)形成細胞的凋亡指數(shù)不存在相關(guān)性(r=0.13,P>0.05),扁平苔蘚組AIF的陽性表達強度與其自身的角質(zhì)形成細胞的凋亡指數(shù)呈正相關(guān)(r=0.64,P<0.01)。結(jié)論AIF可能參與了扁平苔蘚角質(zhì)形成細胞的細胞凋亡,NF-κB可能參與了扁平苔蘚的炎癥反應(yīng)。碩士研究生葛晶(皮膚性病學(xué))指導(dǎo)教師王桂芝教授,王君副教授關(guān)鍵詞:扁平苔蘚;NF-κB;AIF;免疫組化;細胞凋亡摘要AbstractObjectiveTheobj
4、ectiveofthisstudywastoinvestigatetheexpressionandsignificanceofnuclearfactorkappaB(nuclearfactorkappaB,NF-κB)andapoptosis-inducingfactor(apoptosis-inducingfactor,AIF)inlichenplanus(LP).MethodsImmunohistochemistryandTUNEL(Terminaldeoxynucleotidyltransf
5、erase-mediateddUTPNikeEndLabeling)wasusedtodetecttheintegratedopticaldensityandapoptosisindexofNF-κBandAIFinparaffin-embeddedsamplesoflichenplanus(n=25)andnormalskinsamples(n=25).ResultsTheresultsshowthattheintegratedopticaldensityofNF-κBandAIFinLPwer
6、ehigherthanthatinthecontrolgroup(t=3.14and4.09,P<0.01);Keratinocyteapoptosisindexinlichenplanuswassignificantlyhigherthanthatinthecontrolgroup.Statisticallysignificantdifferencesbetweenthetwogroups(t=4.97,P<0.01).Therewasnocorrelationbetweentheexpress
7、ionintensityofNF-κBanditsownapoptosisindex(r=0.13,P>0.05),TheexpressionintensityAIFinlichenplanusgroupwaspositivelycorrelatedwithitsownapoptosisindex(r=0.64,P<0.01).ConclusionsTheexpressionofAIFinthelesionsoflichenplanusmaybeinvolvedinthecellapoptosis
8、excitementofkeratinocytes.NF-κBmaybecloselyassociatedwiththeinflammatoryresponseinlichenplanus.Graduatestudent:JingGe(DermatologyandVenereology)DirectedbyProf.GuizhiWangandProf.JunWangKeywords:lichenplanus;NF-κB;AIF;immunohistochemical;apoptos