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1、內(nèi)蒙古農(nóng)業(yè)大學(xué)碩士學(xué)位論文酵母培養(yǎng)物對(duì)綿羊瘤胃發(fā)酵與生產(chǎn)性能的影響姓名:那日蘇申請(qǐng)學(xué)位級(jí)別:碩士專業(yè):動(dòng)物營養(yǎng)與飼料科學(xué)指導(dǎo)教師:敖長金;桂榮2003.5.1摘要本研究用酵母(聃)菌種,采取菌種液體培養(yǎng)一固體發(fā)酵的方法制備酵母培養(yǎng)物,活菌數(shù)達(dá)到3X109個(gè)/g。產(chǎn)生水解酶類及酶活分別為,B一1,4葡聚糖酶(cx酶):2517,60Utg;總纖維素酶:170.49U/g;半纖維素酶(其中的木聚糖酶)2092.69u/g:中性蛋白酶:20、5.13u/g。不同培養(yǎng)基中CP提高13.30%~18.54%,總氨基酸提高8.57%~11.69%。j、乒4——進(jìn)行了酵母培養(yǎng)物對(duì)放牧補(bǔ)飼綿羊
2、瘤胃發(fā)酵及生產(chǎn)性能影響的對(duì)比實(shí)驗(yàn),按10g/<只’d)添加連續(xù)飼喂30天,提高日增重11.1%(P>0.05)。提高干物質(zhì)消化率:顯著提高粗蛋白質(zhì)消化率達(dá)26.97%(P<0.05);NDF、ADF、半纖維素消化率分別提高4.58%、7.26%、2.86%。顯著降低飼喂后瘤胃內(nèi)氨態(tài)氮的含量(P<0.05)。促進(jìn)揮發(fā)性脂肪酸的生成,提高總揮發(fā)性脂肪酸量和乙酸/丙酸比值。提高飼喂后原蟲數(shù)。提高綿羊血清氨基酸量和血清總蛋白量。穩(wěn)定瘤胃pH值。關(guān)鍵詞:酵母培養(yǎng)物;綿羊;瘤胃;放牧補(bǔ)飼;生產(chǎn)性能、ABstractYeast(JM)anditSculturewerestudied.Yeas
3、tcultureWaspreparedbyliquid.cultivationandsolid—fermentation.CFUWas3x109/ginYeastculture.Inyeastculture,activityofB一1,4glucanaseWas2517.60U/g;activityoftotalcellulasewas170.49U/g;activityofhemicellulase(xylanase)Was2092.69U/g;activityofneutralproteinaseWas205.13U/g.CPWasIncreasedby13.30%~18.
4、54%.totalaminoacidWasincreasedby8.57%~11.69%indifferentculturemedium.TheeffectofYeastcultureonralliesfermentationandproductionperformanceofsheepthatweregrazingandsupplementaryfeedingWasstudied.10gYeastcultureWasaddedinrationofeachsheepperday,testperiodwas30days.Averagedailygainofsheepoftreal
5、raentgroupwasincreased11。1%thanthatofcontrolgroup(P>O。05)。ApparentdigestibilityofDMoftreatmentgroupdietaryWasincreased.ApparentdigestibilityofCPoftreatmentgroupdietaryWasincreasedby26.97%(P6、d2.86%respectively.ConcentrationofNHrNwasdecreasedsigrdficantly(P<0.05),numberofremenprotozoanincreased柵feedingYeastculture.ConcentrationofTVFAWasincreased,AceticaCid/propionicacidwasincreased.Serumaminoacidandtotalproteinwereincreased.RumenpHWassteady.Keywords:Yeastcuffure,Sheep;Rumen;Grazi
7、ngandsupplementaryfeeding;Productionperfor腳nceDirectedby:Prof.GuirongProf.AoChan自inApplicantforMasterdegree:NaRisu(AnimalNutrition∞dF∞dScienee)AnimalScieneeCollege,lmacrMongoliaAgricullatralUnivcrsity,Huhhot010018,China——.一.塑塞查奎些查蘭堡主堡奎11前言從本世紀(jì)祝梅切尼科