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1、BacillusSubtilisExpressionVectorsProductInformationandInstructionsNovember2005B.subtilisExpressionVectors11/20051Content1.Introduction.........................................................................32.ThepHTVectors..................................
2、...............................42.1.VectorMappHT01...........................................................42.2.VectorMappHT43...........................................................52.3.LocationofthetagsinthepHT01derivatives..................63.Protoc
3、ols.............................................................................64.References.......................................................................75.OrderInformation,Shipping&Storage................................7Thesevectorsystemshavebe
4、endevelopedinthelaboratoryofWolfgangSchumannattheInstituteofGenetics,UniversityofBayreuth,Germany.Forresearchuseonly!B.subtilisExpressionVectors11/20052B.subtilisExpressionVectorsPlasmid-basedexpressionvectorsforhighlyefficientintra-andextracellularproducti
5、onofrecombinantproteinsinBacillussubtilis1.IntroductionGram-positivebacteriaarewellknownfortheircontributionstoagricultural,medicalandfoodbiotechnologyandfortheproductionofrecombinantproteins.Amongthem,Bacillussubtilishasbeendevelopedasanattractivehostbecau
6、seofseveralreasons:(i)Itisnon-pathogenicandisconsideredasaGRASorganism(generallyregardedassafe);(ii)ithasnosignificantbiasincodonusage;(iii)itiscapableofsecretingfunctionalextracellularproteinsdirectlyintotheculturemedium(atpresent,about60%ofthecommercially
7、availableenzymesareproducedbyBacillusspecies);(iv)alargebodyofinformationconcerningtranscription,translation,proteinfoldingandsecretionmechanisms,geneticmanipulationandlarge-scalefermentationhasbeenacquired.ButtherearealsotwoobstaclesreducingtheuseofB.subti
8、lis:(i)productionofanumberofextracellularproteaseswhichrecognizeanddegradeheterologousproteins,and(ii)stablevectorplasmids.Thefirstobstaclehasbeenlargelysolvedbytheconstructionofprotease-deficientstrai
