降纖酶對(duì)大鼠腦缺血再灌注后神經(jīng)損傷的保護(hù)作用

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1、鄭碩士論文題目：作者姓名：學(xué)科門類：專業(yè)名稱：導(dǎo)師姓名、職稱：州大學(xué)位授予單位代碼學(xué)號(hào)或申請(qǐng)?zhí)柮芗?jí)學(xué)論文降纖酶對(duì)大鼠腦缺血再灌注后神經(jīng)損傷的保護(hù)作用白蓉醫(yī)學(xué)臨床神經(jīng)病學(xué)張博愛教授2005年10月蘭第l兒鄭州凡學(xué)2【)05J1{；碩}。畢業(yè)論文降纖酶對(duì)大鼠腦缺血再灌注后神經(jīng)損傷的保護(hù)作用研究生自蓉導(dǎo)師張博愛鄭州大學(xué)第一附屬醫(yī)院神經(jīng)內(nèi)科郊卅l450052摘要目的觀察降纖酶對(duì)缺血再灌注大鼠腦損傷的保護(hù)作用。方法采用大鼠腦缺血再灌注動(dòng)物模型，觀察降纖酶對(duì)大鼠腦缺血／再灌注不同時(shí)間點(diǎn)梗死體積、腦血流量、行為學(xué)評(píng)分、凋亡數(shù)目的影響。采用T

2、Tc染色法測量梗死體積、激光多譜勒血流儀監(jiān)測血流量、神經(jīng)功能缺損評(píng)分(Nss)評(píng)估行為學(xué)改變、凋亡數(shù)目的變化使用t岫el方法觀察。結(jié)果與生理鹽水組比較降纖酶組在缺血3h再灌注6h、24h，腦梗死體積減小(P(0．05)，血流增加(P(0．05)，在缺血3h再灌注3h、72h，腦梗死體積與生理鹽水組比較無明顯變化。降纖酶組動(dòng)物行為學(xué)評(píng)分無明顯改善，凋亡細(xì)胞數(shù)目隨再灌注時(shí)間延長而漸增多，於24h達(dá)高峰，以后逐漸減少。降纖酶組凋亡細(xì)胞數(shù)變化趨勢(shì)與對(duì)照組相同，但凋亡細(xì)胞數(shù)明顯減少(P

3、血／再灌注后的低灌注狀態(tài)，挽救損傷神經(jīng)細(xì)胞，減少神經(jīng)細(xì)胞凋亡，減小腦梗死體積，擴(kuò)大腦梗死治療時(shí)間窗，對(duì)腦組織具有一定的保護(hù)作用。關(guān)鍵詞I鞴燃梗嗍腦缺血j撇行為黼凋亡第2負(fù)琊州人學(xué)2005屬壩fj畢業(yè)滄ZTheprotectiVeeffectofde脅船seonthece睫braIischemiareperfusi蚰ⅫuryPostgraugrate：BaiRongTutor；ZhangBouaiTheFirstAffiliatedHospitalofZhengzhOuUniVefsityAbsn·act0bJectiveTbd

4、eterminetheprotectiveeffectofdefibraseonthecerebralischemiareperfusioninjuryintherats．MethodsAtVarioustimepointsinthemodelratsofccrebralischemiareperfusionafterdefibraseadministeredthechangesofcerebralinfarctvolumeweremeasufedwithTTCstain，thecerebralbloodnowwereinsp

5、ectedbymeansofTheDopularBloodflowInstrument，thebehaviorscofeswereperfbmedwithNeurologicalseVefityscores(Nss)testatthetimejustafteroperationandbefbresacrificeandthenumberofapoptosiscellswercexaminedwithTUNELmethod．ResuItsThecerebralinfarctvolumewassjgnificantlydecrea

6、sed(P<0．05)andthecerebralbloodnowwasincreasedwhenreperfusingfor6hand24hafterischemiafor3hindefibrasegroupscomparedwithsalinecontrOlgroups．However'ThcreisnodiffefenceincerebralinfarctVolumebetweendefibrasegroupsandthecontrDlgroupswhenreperfusingfor3hand72hafterischem

7、iafor3h．1ndefibrasegroupsthescaleOfbehaviorwasnotimproVed．TheTUNEL-positiVecellswerepreferentiallylocatedintheinnerbOundaryzoneoftheinfactjonandprogressiVelyincreaseduptoandpeakedat24hafterreperfUsion，andthendecreasedgraudually，Thechangeabletendencyofbraincellapopto

8、sisinthedefibrasegroupshaVealikewithcontrolgroups，thenumberoftheapoptoticcelldecreased(P