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1、BCDEFGH2010+8*-15.-8/ChineseClinicalOncology,Aug.2010,Vol.15,No.8·685·Rrasc?ˉ°w±2{3′μ?AB|§1157011&'()*()*(úaüV+,X-.(,/"0,123【】K{úZRras°42!?#¢?¢L}?f£mRNA1,EOBRras£1?BC?Hz?£i&?±BE?!?=GAPDHmRNA71UfQà1o?SPCRa?SZ??#¢}?§¢L}?fRrasmRNA£1,E?±?BC?Hì
2、£i&。aRr
3、as°?#¢、¢L}?£1?5?g
4、FBE(P<00001);?#¢}?£Rras1?ò????A:、DE$、PR£1??hi?(P<005)。ORras£1e72ü?#¢b=b£=aFz?,±?1??y?!尽?#¢;Qà1o?SPCR;Rras=:R7379j::Aj$=:1009-0460(2010)08-0685-04ExpressionofRrasgeneinbreastcanceranditsclinicopathologicalsignificanceSONGJie,BUXi
5、aobo,XUChunyan.DepartmentofBiology,MudanjiangMedicalCollege,Mudanjiang157011,China【Abstract】ObjectiveToinvestigatetheexpressionofRrasinbreastcancerandtoanalyzetherelationshipbetweenitsexpressionandclinicalpathologicalcharactersandtoexplorethesignifi
6、canceofRrasmRNAexpressioninthecarcinogensisanddevelopmentofbreastcancer.MethodsRealtimequantitativereversetranscriptionpolymerasechainreaction(QRTPCR)techniquewasappliedfordetectingRrasexpressioninthetissuesofbreastcancerandnormaladjacenttumortiss
7、ues.Therelationshipofclinicalandbiologicalparameterswasalsodiscussed.ResultsTheexpressionofRrasmRNAofbreastcancerwaslowerthanthatofnoncanceroustissue(P<00001),showingasignificantdifferencetonormalbreasttissue.Rrasexpressionwasrelatedtolymphnodemetas
8、tasis,tumordiameterandPRexpression(P<005).ConclusionTheexpressionofRrasinbreastcancermightbeausefulparameterasanindicatoroftumormetastasisandcarcinogensis.ThehighexpressionofRrasgeneinbreastcancerprobablyimpliesabetterprognosis.【KeyWords】Breastcance
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