資源描述：
《characterization of a hyperthermostable》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學術(shù)論文-天天文庫。
1、ApplMicrobiolBiotechnol(2007)75:367–376DOI10.1007/s00253-006-0834-3BIOTECHNOLOGICALLYRELEVANTENZYMESANDPROTEINSCharacterizationofahyperthermostableFe-superoxidedismutasefromhotspringYong-ZhiHe&Ke-QiangFan&Cui-JuanJia&Zhi-JunWang&Wu-BinPan&LiHuang&Ke-QianYang&Zhi-YangDongReceived:30O
2、ctober2006/Revised:25December2006/Accepted:28December2006/Publishedonline:30January2007#Springer-Verlag2007AbstractAnewgeneencodingathermostableFe-tion-independentapproaches(extractingDNAdirectlysuperoxidedismutase(tcSOD)wasidentifiedfromafromenvironmentalsamplesandconstructingmetag
3、e-metagenomiclibrarypreparedfromahotspringsample.nomiclibraries)havebeendevelopedtostudytheseTheopenreadingframeoftcSODencodeda211aminolargelyuntappedorganismsandtheirfunctions.Inrecentacidprotein.Therecombinantproteinwasoverexpressedyears,anincreasingnumberofnewgenesencodinginEsche
4、richiacoliandconfirmedtobeaFe–SODwithapotentiallyusefulenzymeshavebeenidentifiedfromspecificactivityof1,890U/mgusingthepyrogallolunculturedmicrobesbycultivation-independentways.method.Theenzymewashighlystableat80°CandTheseincludechitinasefromuncultivatedmarinebacteriaretained50%acti
5、vityafterheattreatmentat95°Cfor2h.(Cottrelletal.1999),lipolyticenzymesfromsoilsamplesItshowedstrikingstabilityacrossawidepHspanfrom4(Henneetal.2000),andthermostableesterasefromhotto11.Thenativeformoftheenzymewasdeterminedasaspringandmudholesamples(Rheeetal.2005).Manyhomotetramerbyan
6、alyticalultracentrifugationandgradi-methodshavebeendevelopedtoscreenametagenomic2+entnativepolyacrylamidegelelectrophoresis.Fewaslibraryforgenesofinterest.ScreeningmaybebasedonfoundtobeimportanttoSODactivityandtothestabilityhybridizationwithaknownprobe,orasimpleenzymeoftcSODdimer.Co
7、mparativemodelinganalysesofassay.However,thesemethodsareofoften-limitedusetcSODtetramerindicatethatitshighthermostabilityisduetotechnicalproblemsordependenceonfortuitousmainlyduetothepresenceofalargenumberofexpressionoffunctionalproteins.High-throughoutendintersubunitionpairsandhydr
8、ogenbondsandtoasequ
