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1、人血清IgG分離純化方法探討2IgGpurificationmethodAccordingtothedifferentcharacteristicsofIgG,thepurificationmethodsofIgGaremainlyasfollows:2.1saturatedammoniumsulfateprecipitationmethodThesaltingoutmethodisoneoftheimportantmethodsofcrudeproteinseparation.Becauseproteinadsorptionsomesalt,withitsmeterlay
2、ersothattheproteinmoleculesrepeleachother,buttheinteractionbetweenproteinandwatermoleculesarestrengthened,thusimprovingthesolubility?Butwhenalargenumberofneutralsaltisaddedtowater,theactivitydecreased,resultinginproteinthemolecularsurfacechargehasbeenneutralized,thewaterfilmisgraduallydest
3、royed,resultinginproteinmoleculesbetweenaggregateandprecipitatefromsolution.Theammoniumsulfateisthemostcommonlyusedinorganicsaltforsaltingout,itsmainfeatureisthelargesolubilitywithsmallchangesintemperature;hasaprotectiveeffectonprotein,highconcentrationcaninhibitmicrobialandproteaseactivit
4、y;dissolvedinwaterdoesnotproduceheat,thepriceislow,butnotinthealkalineenvironmentusingammoniumsulfateasprecipitantforsaltingoutreactioninblood.TheadditionofammoniumsulfateinQingDynasty,whenthesaturationis28、33,euglobulinprecipitation;33~55,pseudoglobulinprecipitation;saturationisgreatertha
5、n50%,whichisammoniumsulfateprecipitationofalbumin,[3]?analysisofgeneralrulesofserumproteinprecipitation2.2organicsolventprecipitationmethodOrganicsolventprecipitationmethodistodestroytheformationofsolutemoleculesaroundthehydrationlayerbyorganicsolvent,thesolutedehydrationandaggregationofpr
6、ecipitation,orreducesthesolubilityofthesolute;andthedielectricconstantoforganicsolventsthanwater,withtheadditionoforganicsolvent,reducingthedielectricconsta.ntofthesolution,theCoulombicattractionbetweenthechargedsolutemoleculesgraduallyincreased,andthemutualattractionandaggregation.Ingener
7、al,thegreatertheamountofsolutemoleculesmoreeasily,organicsolventprecipitation,organicsolventconcentrationprecipitationtothelower[7]?inthismethod?Mainlyintroducesthebitterness2.3polyethyleneglycol(PEG)replacementmethodWatersolublenonionicpolymerpolymerprecipita
