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1、齊齊哈爾大學(xué)畢業(yè)設(shè)計(jì)（論文）題n低劑量微波輻射對(duì)重金屬污染下玉米幼苗生長(zhǎng)發(fā)育的影響學(xué)院生命科學(xué)與農(nóng)林學(xué)院專業(yè)班級(jí)生物技術(shù)083班學(xué)生姓名賀曉杰指導(dǎo)教師吳旭紅教授成績(jī)2012年6月20口摘要對(duì)浸泡10h后的種子分別釆取Os,5s,10s,15s,20s的低火微波處理(微波強(qiáng)度為180mW/cm2)o用盆栽的方法培養(yǎng)玉米幼苗，7d后測(cè)定發(fā)芽率，lid后，測(cè)發(fā)芽指數(shù)和活力指數(shù)。待玉米長(zhǎng)至三葉期后，每天早晚用注射器滴灌5mLCdCl?溶液，溶液設(shè)低、中、高3個(gè)濃度梯度,即0.01,0.1,1.0mmol/L
2、,對(duì)照組(CK)澆等量自來(lái)水,3d后釆集玉米葉片測(cè)定生理指標(biāo)。與對(duì)照組相比較，低劑量輻射可以提高玉米種子發(fā)芽率，高劑量輻射降低發(fā)芽率，4種不同時(shí)間長(zhǎng)度的處理均可以提高蛋白酶的活性，經(jīng)過(guò)高濃度CdCl?處理的玉米幼苗葉片屮的超氧化物歧化酶(SOD)和過(guò)氧化物酶(POD)等酶的活性低于對(duì)照組，蛋白質(zhì)的含量低于對(duì)照組，葉綠素a、b的含量明顯低于對(duì)照。這表明，用適宜劑量的微波輻射處理玉米種子能提高玉米的發(fā)芽率，有助于玉米幼苗的生長(zhǎng)發(fā)育，低濃度的鎘溶液能夠促進(jìn)玉米幼苗中S0I)和P0I)的活性，高濃度的鎘溶液
3、對(duì)其產(chǎn)生抑制作用。對(duì)葉綠素的影響則表現(xiàn)為低濃度的鎘對(duì)葉綠素a和葉綠素b均有刺激作用，高濃度的鎘對(duì)其則起到了抑制作用。關(guān)鍵詞：玉米幼苗；微波輻射；氯化鎘；發(fā)芽指數(shù)；蛋口質(zhì)含量；幼苗發(fā)育AbstractSoaktheseedsafterlOh,respectively,totaketheOs,5s,10s,15s,20s,thelow-firemicrowavetreatment(microwaveintensityof180mW/cm2).Potmethodtocultivatemaizeseedli
4、ngs,measuredaftergerminationrate7d,lid,measuredgerminationindexandvigorindex.Cornuptothree-leafstageuntilafterday,morningandeveningdripirrigationwithasyringe5mLofCdChsolution,thesolutionsetlow,mediumandhighconcentrationgradient3,namely0.01,0.1,1.0mmol/
5、L,controlgroup(CK),pouringthesameamounttapwater,collected3daftercornleavesdeterminationofphysiologicalindicators.Comparedwiththecontrolgroup,lowdosesofradiationcanbeimprovedmaizeseedgerminationrate,high-doseradiationtoreducethegerminationrate,fourdiffe
6、rentlengthoftimeprocessingcanimprovetheactivityoftheprotease,superoxideafterthetheCdChprocessingofthehighconcentrationofleavesofmaizeseedlingstheenzymesuperoxidedismutase(SOD)andperoxidase(POD)activitywaslowerthaninthecontrolgroup,theproteincontentislo
7、werthanthecontrolgroup,chlorophyllaandbweresignificantlylowerthanthecontrol.Thissuggeststhatappropriatedosesofmicrowaveradiationprocessingofcomseedlingscanimprovecorngerminationrate,contributetothegrowthanddevelopmentofmaizeseedlings,andlowconcentratio
8、nsofcadmiumsolutioncanpromotetheactivityofSODandPODinmaizeseedlings,highconcentrationsofcadmiumsolutionproducedinhibition.Thechlorophyllperformancelowconcentrationsofcadmiumonchlorophyllaandchlorophyllbhaveastimulatingeffect,highconcent
