資源描述：
《冠狀病毒Coronavirusl論文-1996 Mucosal immunity_ an overview and studies of enteric and respiratory coronavirus infections in a swine model of ent.pdf》由會(huì)員上傳分享，免費(fèi)在線(xiàn)閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、VeterinaryimmunologyandVeterinaryImmunologyandImmunopathologyimmunopathokgyELSEVIER54(1996)163-169Mucosalimmunity:anoverviewandstudiesofentericandrespiratorycoronavirusinfectionsinaswinemodelofentericdiseaseLindaJ.SaifFoodAnimdHealthResearchProgrum,DepartmentofVeterin
2、aryPreventiveMedicine.OhioAgriculturalReseurchundDevelopmentCenter,TheOhioStuteUniversity,Wooster,OH44691.USAAbstractBasedonthetenetofacommonmucosalimmunesystem,antigenicstimulationatonemucosalsiteresultsinthedistributionofantigen-specificIgAprecursorcellstodistantmuc
3、osalsites.However,recentstudiessuggestthatfunctionalcompartmentalizationandlimitedreciprocitymayexistwithinsomecomponentsofthecommonmucosalimmunesystem.Althoughoralimmuniza-tionisoftenveryeffectiveininducingimmunitytorespiratorypathogens,theconverse(respira-toryimmuni
4、zationtoprevententericdiseases)maynotbeaseffective.Toaddressthisquestionandtostudyinteractionsbetweenthebronchus-associated(BALT)andgut-associated(GALT)lymphoidtissuesrelatedtoprotectiveimmunity,weusedasamodeltwoantigenicallyrelatedporcinecoronaviruseswhichreplicatepr
5、imarilyintheintestine(transmissiblegastroenteritisvirus,TGEV)orrespiratorytract(porcinerespiratorycoronavirus,PRCV).Thetissuedistributionandmagnitudeoftheantibodysecretingcell(ASC)responses(measuredbyELISPOT)andcell-medi-atedimmuneresponses(measuredbylymphoproliferati
6、veassays,LPA)coincidedwiththeviraltissuetropisms.ImmunizationviaGALT(gutinfectionwithTGEV)elicitedhighnumbersofIgAASCandhighLPAresponsesinGALT(gutlaminapropria,LPormesentericlymphnodes,MLN),butlowerresponsesinBALT(bronchiallymphnodes,BLN)andinducedcompleteprotectionag
7、ainstentericTGEVchallenge.IncontrastimmunizationviaBALT(respiratoryinfectionwithPRCV)elicitedsystemictyperesponses(highnumbersofIgGASCintheBLN),butfewASCandlowLPAresponsesinthegutLPorMLNandinducedonlypartialprotectionagainstentericTGEVchallenge.Thusadministrationofvac
8、cinesintranasallymaynotbeoptimallyeffectiveforinducingintestinalimmunityincontrasttothereportedefficacyoforalvaccinesforindu
