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1、VECTOR-BORNEANDZOONOTICDISEASESSHORTCOMMUNICATIONVolumeXX,NumberXX,2016aMaryAnnLiebert,Inc.DOI:10.1089/vbz.2016.2062HighPrevalenceofMiddleEastRespiratoryCoronavirusinYoungDromedaryCamelsinJordan12345NeeltjevanDoremalen,ZaidounS.K.Hijazeen,PeterHolloway,BilalAlOmari,
2、ChesterMcDowell,643789DanielleAdney,HaniA.Talafha,JavierGuitian,JohnSteel,NadimAmarin,MarkosTibbo,EhabAbu-Basha,4AhmadM.Al-Majali,4VincentJ.Munster,1,*andJuergenA.Richt5,*AbstractPrevalenceofMiddleEastrespiratorysyndromecoronavirus(MERS-CoV)wasdeterminedin45dromedar
3、ycamelsfromtwogeographicallyseparatedherdsinJordan.Virussheddingwasonlydetectedinswabsobtainedfromtherespiratorytractandprimarilyobservedincamelsyoungerthan3years.MERS-CoVseroprevalenceincreasedwithageofcamels.Bovineandsheepserawereseronegative.Phylogeneticanalysiso
4、fpartialS2clusteredtheJordanianMERS-CoVstrainswithcontemporaryMERS-CoVstrainsassociatedwithnosocomialoutbreaks.Keywords:dromedarycamel,Jordan,MERS-CoV,phylogeny,serologyIntroductiontwolocationsinJordan.Twocamelherdswereidenti?edtostudy(1)atraditionalbedouincamelherd
5、,inwhichcamelsSincetheidenti?cationofMiddleEastrespiratoryareallowedtograzeandbrowsefreely(Azraq,Zarqa)and(2)syndromecoronavirus(MERS-CoV)asthecausativeamoreconventionalmixedfarmsetting,wherecamelswereagentofafatalcaseofrespiratorytractdiseaseintheKingdomkeptinpenso
6、nonefarm(Ramtha)(Fig.1A).InRamtha,ofSaudiArabia(KSA)in2012,thevirushascaused>1700bloodsamplesfromadultsheepandcattlewereadditionallylaboratory-con?rmedcasesofdisease,including>600fatalcollected.Swabsampleswerecollectedinvirustransportcases(WHO2016).The?rstknownMERS-
7、CoVoutbreakinmedium.RNAwasextractedfromsamplesusingthehumans,diagnosedretrospectively,occurredinJordanin2012QiaAmpViralRNAkit(Qiagen).FivemicrolitersofRNA(Hijawietal.2013).Thedromedarycamelhasbeenidenti?edwasusedinaone-stepreal-timeRT-PCRUpEassayforastheprimaryreser
8、voirofMERS-CoV,anddirectevidenceforMERS-CoVusingtheRotor-Gene?probekit(Qiagen).zoonotictransmissionfromcamelshasbeenreportedinKSAPositives
