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1、StudiesWithHumanCoronaviruses11.SomePropertiesofStrains22YEandOC43(36224)ROBERTA.BUCKNALL,~LULAhl.KING,ALBERTz.KAPIKIAN,ANDROBERTM.CHANOCKLaboratoryofInfectiousDiJeases,NationalInstituteofAllergyandInfectioiuDiseases,NationallnslitiitesofHealth,Bethesda,
2、Maryland20014Thehumancoronavirusesfallintotwoma-notinvolvedintheformationofthecoronavi-jorgroups:thosewhichwereoriginallyiso-ruses(14-16),cellsinfectedwithOC43hem-latedinorganculturesofhumanembryonicadsorbratandmouseredbloodcells(17).trachea(HET)(“obliga
3、teorganculture”Thepurposeofthispresentstudywastogroup)andthosewhichwererecoveredininvestigatesomeofthephysicalandbiologi-humanembryonicmonolayertissuecultures.calpropertiesoftissuecultureadaptedOC43Thelatterconsistsofstrain229E(1)and13andcomparethemwitht
4、hoseof2293.closelyrelatedstrains(1,2)andtheformerMaterialsandMethods.Viruses.Coronavi-ofninestrains(3-6)includingtwo(OC38rus229EwasobtainedfromDr.D.Hamreofand003)whichareknowntobeidentical,theUniversityofChicago,Illinoisandtissueandone(LE’)whichisclosely
5、relatedtoculturepoolsusedinthisstudywerederivedstrain229E(7-10).Despiteefforts(3-6,11,fromthisstrain.OC43virusadaptedto12)toadaptmembersoftheobligateorgangrowinhumandiploidcellstrain(HDCS)culturecoronavirusgrouptotissueculture,WI38cultureswasavailableint
6、hislaborato-onlystrainsB814,(thefirstdescribedcorona-ry(17)andbothvirusesweregrownvirusofhumanorigin),LP,EVS,OC43(andinHDCSWI38culturesmaintainedonaOC38)havebeensuccessfullygrownincon-mixtureofhalfEagle’sMinimalEssentialventionaltissueculturecells(6,11,1
7、2).MediuminEarle’sBSSandhalfMediumAlthoughstrainOC43showsatypical199,containing2%fetalcalfserum,or0.2%coronavirusstructureintheelectronmicro-bovineplasmaalbumin(ArmourfractionV),scope,thevirushasseveralbiologicalcharac-100U/mlpenicillinand100pg/mlstrepto
8、-teristicsotherthanitsserologicalreactions,mycin.whichdistinguishitfromtheotherhumanVimsassay.229EandOC43wereassayedcoronaviruses.inHDCSW138cells:229EbyCPEand1.VirusgrowninHETculturecausesanOC43byhemadsorption.Hemadsorptio
