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《氟吡汀對(duì)神經(jīng)病理性疼痛大鼠的鎮(zhèn)痛作用及其機(jī)制研究.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在工程資料-天天文庫(kù)。
1、中國(guó)疼痛醫(yī)學(xué)雜志ChineseJournalofPainMedicine2014．20(4·213·doi：10．3969~．issn．1006—9852．2014．04．006氟吡汀對(duì)神經(jīng)病理性疼痛大鼠的鎮(zhèn)痛作用及其機(jī)制研究烏云爾德尼翁澤林馮濤張小沼(華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬協(xié)和醫(yī)院疼痛科，武漢430022)摘要目的：探討鉀離子通道開(kāi)放劑氟吡汀對(duì)神經(jīng)病理性疼痛大鼠的鎮(zhèn)痛作用及交感芽生的影響，為神經(jīng)病理性疼痛藥物治療提供理論依據(jù)。方法：成年雄性SD大鼠18只，體重250～350g，按隨機(jī)數(shù)字表法隨機(jī)分為3組，每組6只：假手術(shù)組(S組)，對(duì)照組(C組)，氟
2、"kliT組(F組)。s組大鼠暴露腰5(L)脊神經(jīng)，但不結(jié)扎。其余組大鼠暴露k脊神經(jīng)并結(jié)扎，建立疼痛模型。s組和C組大鼠腹腔注射生理鹽水2ml／kg，F(xiàn)組大鼠腹腔注射氟吡汀5m~／kg，并連續(xù)給藥7d。各組大鼠術(shù)前3d及術(shù)后1、3、5、7d檢測(cè)機(jī)械痛閩(pawwithdrawalpressurethreshold，PWPT)和縮足反射潛伏期(pawwithdrawallatency,PwL)。術(shù)后第7d，各組大鼠取手術(shù)側(cè)L5背根神經(jīng)節(jié)采用免疫熒光染色標(biāo)記法檢測(cè)背根神經(jīng)節(jié)內(nèi)籃狀結(jié)構(gòu)變化。結(jié)果：與s組比較，C組大鼠PWPT和PWL術(shù)后第1d起顯著降低(P<0．
3、05)，維持至術(shù)后第7d。與C組比較，F(xiàn)組大鼠術(shù)后第3d起PWPT和PWL明顯提高，差異有統(tǒng)計(jì)學(xué)意義(P<0．05)。與C組比較，經(jīng)過(guò)氟吡汀治療后，F(xiàn)組大鼠術(shù)后第7d手術(shù)側(cè)L背根神經(jīng)節(jié)內(nèi)籃狀結(jié)構(gòu)顯著減少(P<0．05o結(jié)論：氟吡汀通過(guò)抑制背根神經(jīng)節(jié)內(nèi)交感芽生以減輕神經(jīng)病理性疼痛。關(guān)鍵詞神經(jīng)病理性疼痛；氟吡??；交感芽生；背根神經(jīng)節(jié)ANALGESICEFFECTS0重’’LUPIRITlE0SPINALERVELIGArl’lU-IDUCEDNEURoPATHICPAININRATSWU．Yun．Er-De—Ni，WENGZe．Lin，F(xiàn)ENGTao，ZHAN
4、GXiao．Ming△(DepartmentofPain，UnionHospital，TongjiMedicalCollege，HuazhongUniversityofScienceandTechnology,Wuhan430022)AbstractObjective：Toinvestigatetheeffectsofflupirtineonneuropathicpainandsympatheticsproutinginthedorsalrootganglionofratsafterspinalnerveligation(SNL)．Methods：18he
5、althyadultmaleSprague·-Dawleyratsweighing250——350gwererandomlydividedintothreegroupswithsixratsineachgroup，includingshamgroup(groupS)，controlgroup(groupC)andflupirtinegroup(groupF)．TheneuropathicpainmodelwasestablishedbytheL5spinalnerveligation．IngroupS，theL5spinalnervewasexposedb
6、utnotligated．IngroupCandF，theL5spinalnervewasexposedandligated．TheratsingroupSandCreceivedsaline2ml／kgandtheratsingroupFreceivedflupirtine5mg／kgviaintraperitonealinjectiondailyforsevendaysafterthesurgery．Painthresholdwasestimatedbymeasuringpawwithdrawalpressurethreshold(PWPT)andla
7、tency(PWL)3daysbeforesurgery(baseline)and1，3，5，7daysaftersurgery．Theratsweresacrificedonthe7thdayaftersurgeryandtheL5dorsalrootganglionofallratswerecollectedtoobservethechangeofbasketformationbyimmunofluorescentstaining．Results：PWPTandPWLingroupCweresignificantlylowerthanthoseingr
8、oupSwithin1～7daysaftersurgeryfP<0
