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1、第33卷第3期大豆科學V01.33No.32014鉦6月S0YBEANSCIENCEJun.2014轉凹基因大豆株系的抗旱性評價王興宇,魏崍,王偉威,薛永國,唐曉飛,吳廣錫,王鵬飛,于志遠,劉麗君(1.東北農(nóng)業(yè)大學生命科學學院,黑龍江哈爾濱150030;2.黑龍江省農(nóng)業(yè)科學院大豆研究所,黑龍江哈爾濱150086)摘要:干旱脅迫條件下考察轉AtCBF4基因大豆株系的光合特性(凈光合速率、氣孔導度、胞間CO:濃度、蒸騰速率、瞬時水分利用率)、生理特性(脯氨酸、丙二醛、可溶性糖)以及產(chǎn)量性狀(株高、節(jié)數(shù)、單株莢數(shù)、單
2、株粒數(shù)、百粒重)的表現(xiàn),綜合評價7個株系之間的抗旱性差別。結果表明:轉AtCBF4基因大豆葉肉細胞中脯氨酸的含量和可溶性糖含量與受體相比較高,丙二醛含量增幅較小,光合生產(chǎn)能力和產(chǎn)量性狀表現(xiàn)優(yōu)于受體。綜合結莢期和鼓粒期表現(xiàn),所有轉基因株系耐旱性均強于非轉基因受體,其中HTCB59-5、HTCB59-2和HTCB59-4具有較強的耐旱能力。關鍵詞:大豆;AtCBF4;轉基因株系;抗旱性中圖分類號:$565.1文獻標識碼:ADOI:10.11861/j.issn.1000-9841.2014.03.0365Droug
3、htResistanceEvaluationofAtCBF4TransgenicSoybeanWANGXing—yu,WEILai,WANGWei—wei,XUEYong.guo,TANGXiao—fei,WUGuang—xi,WANGPeng—fei。YUZhi—yuan,LIULi-jun(1.CollegeofLifeScience,NortheastAgrieulturalUniversity,Harbin150030,China;2.SoybeanResearchInstitute,Heilongji
4、angAcademyofAgriculturalSciences,Harbin150086,China)Abstract:Inthispaper,weexploredtheperformanceoftransgenicsoybeansinphotosyntheticcharacteristicsofsoybean(netphotosyntheticrate,stomatalconductance,C0,concentrationintercellular。transpirationrate,instantane
5、ouswateruseefficien—cy),physicalcharacteristics(Pro,MDA,solublesugar)andyieldtraits(plantheight,numberofsections,thenumberofpodsperplant,seednumberperplant,seedweight)underdroughtstress.Comprehensiveevaluatethediferenceindrought·toleranceamong7soybeanlines.C
6、omparedoftransgeniclinesandnormallines,theresearchshowedthatthecontentsofsolublesugar(WSS)andfreeproline(Pro)fromtransgeniclineswerehigherthannormalones,whilelessincrementofMalondialdehyde(MDA)appeared.Thetransgeniclineshadlittlechangeinphotosyntheticcharact
7、eristicsandyieldtraitsfrombeforetounderdroughtresistance.A11aboveshowedthatcomparedwiththenorma1.a(chǎn)lltransgenicsoybeanshavemoreadjustmentforwaterdeficitinpodstageandpod-fillingstage.HTCB59-5,HTCB59-2andHTCB59-4havemoredroughttolerancethanothers.Keywords:Soybe
8、an;AtCBF4;Transgenicstrains;Droughtresistance近年來,東北地區(qū)干旱頻繁,年降水量季節(jié)分基因COR基因的表達,從而提高植物抵抗低溫、干布不均,尤其是在早春時節(jié)春旱普遍存在J。大豆旱及高鹽的能力剖。目前國內外對于轉AtCBF4是對水分敏感的豆科植物,水分的缺失會導致大豆基因的大豆研究很少。因此,對轉AtCBF4基因大減產(chǎn)甚至是絕產(chǎn),因此培育耐旱的