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《甘薯套作對根際土壤細(xì)菌群落多樣性及植株氮素吸收的影響-論文.pdf》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、作物學(xué)報ACTAAGRONOMICASINICA2012，38(2)：333—343http：／／www．chinacrops．org／zwxb／ISSN0496—3490；CODENTSHPA9E—mail：xbzw@chinajournaLnet．cnDoI．10．3724／SP．J．1006．2012．00333小麥／T-．米／大豆和小麥／t米／甘薯套作對根際土壤細(xì)菌群落多樣性及植株氮素吸收的影響雍太文楊文鈺向達(dá)兵朱貞穎四川農(nóng)業(yè)大學(xué)農(nóng)學(xué)院，四川溫江611130摘要：為探討小麥／玉米／大豆和小麥／玉米／甘薯套作體系中根際細(xì)菌群落多樣性與作物氮素高效吸收的差異特性及二者間
2、的關(guān)系，應(yīng)用變性梯度凝膠電泳(DGGE)技術(shù)研究了小麥一大豆(A1)、小麥一甘薯(A2)、玉米(A3)、小麥／t米／大豆(A4)和小麥／t米／甘薯(A5)5種種植模式的根際細(xì)菌群落多樣性。結(jié)果表明，與A1、A2、A3及A5相比，A4套作提高了各作物在開花期(或吐絲期)與成熟期的籽粒吸氮量、地上部總吸氮量和Shannon—Weinerindex多樣性指數(shù)(H)。處理間的吸氮量與日的變化規(guī)律為套作>單作、大豆茬口>甘薯茬口，以A4處理最高。不同種植模式下DGGE圖譜條帶的數(shù)量及亮度有較大區(qū)別，且有幾條特征性條帶發(fā)生了明顯變化。不同種植模式間的細(xì)菌群落結(jié)構(gòu)相似性較低，群落相似度系
3、數(shù)()表現(xiàn)為套作與套作間>套作與單作間；A4與A5間的相對較小，二者間的細(xì)菌群落結(jié)構(gòu)差異較大。A4模式有利于提高根際細(xì)菌群落多樣性，增強(qiáng)植株對氮素的吸收能力。關(guān)鍵詞：小麥／t米／大豆套作；氮素吸收；細(xì)菌群落多樣性；PCR—DGGEEffectofWheat／Maize／SoybeanandWheat／Maize／SweetPotatoRelayStripIntercroppingonBacterialCommunityDiversityofRhizosphereSoilandNi-trogenUptakeofCropsYONGTai—Wen，YANGWen—Yu，XIANG
4、Da．Bing，andZHUZhen—YingAgronomyCollege，SichuanAgriculturalUniversity,Wenjiang611130，ChmaAbstract：Theaimofthisstudywastounderstandtherelationshipbetweendiversityofrhizospherebacterialcomr0unityandnitrogenuptakeofcropsintworelaystripintercroppingsystems：wheat／maize／soybearlandwheat／maize／swe
5、etpotato．Weana-lyzedthediversitiesofrhizospherebacterialcommunityinfivecroppingsystemsusingdenaturinggradientgelelectrophoresis(DGGE)basedon16SrDNA．Thecroppingsystemsincludedwheat-soybean(A1)，wheat-sweetpotato(A2)，maizesinglecrop—ping(A3)，wheat／maize／soybearl(A4)，andwheat／maize／sweetpotato
6、(A5)．Comparedtothesolecroppingsystems(A1，A2，andA3treatments)，theA4treatmentshowedincreasesingrainnitrogenuptakeandtotalnitrogenuptakeamountsofabovegroundofcropsatbothflowering(orsilking)andmatnritystages，andtheShannon—Weinerindicesforrhizospherebacterialcommunitydiversitywasalsoincreasedsi
7、gnificantly．ThevaluesofnitrogenuptakeandtheShannon—Weinerindexintherelaystripinter-croppingsystemsweregreaterthanthoseinsolecroppingsystems．a(chǎn)ndthevaluesinthetreatmentwjtl1soybearlstubbleweregreaterthanthoseinthetreatmentwithsweetpotatostubble．Thenumberandstren
