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1、福建農(nóng)業(yè)學(xué)報29(5):492~497,2014FujianJournalofAgriculturalSciences文章編號:1008—0384(2014)05—492—06鄭梅霞,陳崢,劉波,等.解幾丁質(zhì)類芽胞桿菌幾丁質(zhì)酶活性及其次生代謝物的測定EJ].福建農(nóng)業(yè)學(xué)報,2014,29(5):492-497.ZHENGM—X.CHENZ,LIUB,etaI_TheCaracteristicsofCitinaseAtivityandScondaryMtabolitesinPaenibacillusCitinolyticus[J]FujianJo
2、urnalofAgriculturalSciences,2014,29(5):492—497.解幾丁質(zhì)類芽胞桿菌幾丁質(zhì)酶活性及其次生代謝物的測定鄭梅霞,陳崢,劉波,劉程程。,車建美,藍(lán)江林,潘志針,朱育菁,唐建陽(1.福建省農(nóng)業(yè)科學(xué)院農(nóng)業(yè)生物資源研究所,福建福州350003;2.福建農(nóng)林大學(xué)生命科學(xué)院,福建福州350002)摘要:采用幾丁質(zhì)酶培養(yǎng)基篩選出FJAT一10038芽胞桿菌,測定其幾丁質(zhì)酶活力為11.O4U·mL~,說明FJAT一10038芽胞桿菌能夠產(chǎn)幾丁質(zhì)酶。采用氣相色譜質(zhì)譜聯(lián)用(GC/MS)的方法分析FJAT~10038發(fā)酵液丙
3、酮萃取的成分,初步鑒定出31種化合物。在鑒定出的化合物中,相對含量超過1O%的有2種,分別是六氫化一吡咯環(huán)[1,2一a]一毗嗪一1,4一二酮和異丁醚,相對含量分別為44.17和15.58;匹配率在9O以上的成分有3種,分別是二叔丁基對甲酚、六氫化一吡咯環(huán)[1,2~a]一吡嗪一1,4-二酮和六氫一3一苯甲基一吡咯并[1,2一a]一吡嗪一1—4一-N,匹配度分別是9O、97和93,相對含量分別是0.43、44.17和0.38;另外還發(fā)現(xiàn)一些其他的活性物質(zhì)。關(guān)鍵詞:幾丁質(zhì)酶;氣相色譜質(zhì)譜聯(lián)用;解幾丁質(zhì)類芽胞桿菌;丙酮萃取中圖分類號:S646文獻(xiàn)標(biāo)識
4、碼:ATheCaracteristicsofCitinaseAtivityandScondaryMtabolitesinPaenibacillusCitinolyticusZHENGMei—xia,CHENZhen,LIUBo,LIUCheng-cheng,CHEJian-mei,LANJiang-lin,PANZhi—zhen,ZHUYu—jing,TANGJian—yang(1.AgriculturalBioresourceResearchInstitute,F(xiàn)ujianAcademyofAgriculturalSicences,F(xiàn)uzh
5、ou,F(xiàn)ujian350003,China;2.CollegeoJ’LifeScience,F(xiàn)ujianAgricultureandForestryUniversity,F(xiàn)uzhou,F(xiàn)ujian350002,China)Abstract:ThechitinaseactivityofPaenibacilluschitinolyticusFJAT-10038wascharacterizedbyfilteringFJAT一10038Bacillususingchitinasemedium,thechitinaseactivityofwhichre
6、achedat11.044UmL~.Furthermore,AcetoneextractsofFJAT一10038BacilluswereanalyzedbyGC-MS.Ourresultssuggestthatthirteen-onecompoundsareidentified,andthecontentofPyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro—andPropane,1一ethoxy一2一methyl—reached44.1672and15。5814.Meanwhile,thematchdeg
7、reeofButylatedHydroxyt01uene,Pyrrolo[1,2一a]pyrazine一1,4dione,hexahydro—andPyrrolo[1,2-a]pyrazine一1,4-dione,hexahydro一3一(phenylmethy1)一were90;97and93,witharelativeamountby0.4305,44.1672and0.3842.Inaddition,someotheractivesubstanceswerealsobeencharacterized.Keywords:chitinase
8、;GC/MS;Penibacilluschitinolyticus;acetoneextract2004年Jung—SookLee等Ⅲ通過DNA雜交的菌產(chǎn)幾丁質(zhì)酶的