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1、萬方數(shù)據(jù)新疆農(nóng)業(yè)科學(xué)201l,48(12):2337—2342XinjiangAgriculturalSciencesDOI:CNl(I:65一1097/S.20120109.2104.030網(wǎng)絡(luò)出版時(shí)間:2012—1—921:04:53網(wǎng)絡(luò)出版地址:http://www.cnki.ne∥kcms/detaiL/65.1097.s.20120109.2104.030.htrnl植物根際促生細(xì)菌(PGPR)分離篩選與鑒定楊蓉1,一,房世杰3,楊文琦1,侯敏1,詹發(fā)強(qiáng)1,侯新強(qiáng)1,張慧濤1,龍宣杞1,崔衛(wèi)東1(1.新疆農(nóng)業(yè)科學(xué)院微生物應(yīng)用研究所,烏魯木齊830091
2、;2.新疆農(nóng)業(yè)大學(xué)農(nóng)學(xué)院,烏魯木齊8300523.新疆農(nóng)業(yè)科學(xué)院科研管理處。烏魯木齊830091)摘要:【目的】從不同作物根際土壤中篩選對(duì)黃瓜和番茄有明顯促生效應(yīng)的植物根際促生細(xì)菌(PGPR)。【方法】從番茄、黃瓜、茄子、辣椒四種蔬菜作物的根際土壤中進(jìn)行分離,測定菌株的促生能力,獲得優(yōu)勢廣適菌株,再根據(jù)菌體形態(tài)、培養(yǎng)特征及16srDNA部分序列分析進(jìn)行鑒定?!窘Y(jié)果】共分離得到24株細(xì)菌菌株,經(jīng)平皿初篩,分別篩選出對(duì)黃瓜幼苗、番茄幼苗生長有顯著促生作用的細(xì)菌11和5株,再經(jīng)營養(yǎng)缽促生實(shí)驗(yàn)篩選出2株對(duì)黃瓜、番茄幼苗均具有顯著促生作用的PGPR菌,經(jīng)16srDNA序列
3、分析,分別為芽孢桿菌屬(B0c刪w)和布克霍爾德氏菌屬(曰“以危02如^口)?!窘Y(jié)論】所篩選細(xì)菌對(duì)黃瓜和番茄幼苗有顯著促生作用,為進(jìn)一步構(gòu)建PGPR廣適菌群提供了菌株資源。關(guān)鍵詞:植物根際促生菌;分離;篩選;鑒定中圖分類號(hào):S188+.2文獻(xiàn)標(biāo)識(shí)碼:A文章編號(hào):1001—4330(2011)12—2337—06SievingandIdentificationofPlantGrowth—PromotingRhizobacteriaYANGRon91?,F(xiàn)ANGShi—jie3,YANGwen—qil,HOUMinl,zHANFa—qian91,HOUXin—Qia
4、n91,ZHANGHui—ta01,LONGXuan—qil,CUI礴龜i—don91(1.尺邪eorc^瓜t西M拋Q廠Appf據(jù)d施cr06iofog,,,X吮面口ngAc口如,nyQ廠Ag噸u如“r口fJsc如nc船,Umm咖830091,C危in口;2.C02垤eQ廠Agronow,施研口ngAg^cuffHm2‰眈您妙,m刪i830052,C危in口;3.覷彬口增Acn如,r沙礦Ag“cMf£Mr。fSci饑c∞,昕“m9i830091)Abstract:【Objective】Thepurposeofthisprojectwastoscreenoutef
5、kctiVeplantgrowm—promotingrhizobacteriastrains(PGPR)forcucumberandtomatof.romdifrerentvegetablerhizospheresoil.【Method】PGPRstrainswereisolatedf而mrhizospheresoiloftomato,cucumber,eggplantandcapsiculI∥chiUibasedongmwtll一promotingef島ctinVest遠(yuǎn)ationbypetridishpreliminaryscreeningandnutrit
6、ionpotexperiment.Theselectedemcientandbroadadaptivestrainswerethenidentifiedbymorphological,physiochemical,and16SrDNAanalysis.【Result】24strainsofPGPRwerescreenedprimarily,and11strainsand5strainsofthemcouldobviouslypromotethegmwthofcucumberandtomatorespec“vely.2stminsofthemwerepmVedto
7、beemcientforbothvegetablebynutritionpotexperiment,andweresubsequentlyidentifiedasB口c跏凇sp.a(chǎn)ndBur后^oz如訂口sp.respectively.【conclllsion】The2screenedstrainscouldremarkablypromotetllegrowthofcucumberandtomatoseedlingswhichcouldbestrainmaterialforbroadadaptiVePCPRlibrary.Keywords:plantgmwth—
8、promotingrhi