姜黃素抑制人宮頸癌CaSki細(xì)胞裸鼠移植瘤的生長.pdf

《姜黃素抑制人宮頸癌CaSki細(xì)胞裸鼠移植瘤的生長.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。

1、2015年2月中國比較醫(yī)學(xué)雜志February，2015第25卷第2期CHINESEJOURNALOFCOMPARATIVEMEDICINEVo1．25No．2姜黃素抑制人宮頸癌CaSki細(xì)胞裸鼠移植瘤的生長劉冬菊，姚宇，黃文革(1．廣州中醫(yī)藥大學(xué)第三附屬醫(yī)院，廣州510360；2．中山大學(xué)實(shí)驗(yàn)動(dòng)物中心，廣州510080)【摘要】目的探討姜黃素對(duì)人官頸癌CaSki細(xì)胞裸鼠皮下移植瘤生長的抑制作用。方法建立人宮頸癌裸鼠皮下移植瘤模型，35只荷瘤裸鼠隨機(jī)分為5組，姜黃素高劑量(200mg／kg)、中劑量(100ms／kg)、低劑

2、量(50ms／kg)組、溶劑對(duì)照組和順鉑組(3mg／kg)，每組7只，姜黃素連續(xù)灌胃給藥15d，順鉑組每3d腹腔注射給藥，共5次。停藥24h后處死裸鼠，測量裸鼠體重、移植瘤體積、瘤重，計(jì)算抑瘤率；光鏡下觀察移植瘤組織形態(tài)學(xué)變化；結(jié)果處理組的移植瘤體積均明顯小于空白對(duì)照組(P

3、R33【文獻(xiàn)標(biāo)識(shí)碼】A【文章編號(hào)】1671-7856(2015)02-0007-04doi：10．3969．j．issn．1671．7856．2015．002．002Inhibitoryeffectofcurcuminonthegrowthofhumancervicalcarcinoma(CaSki)xenograftsinnudemiceLIUDong—ju，YAOYu，HUANGWen．ge(1．theThirdAfiliatedHospitalofGuangzhouUniversityofTraditionalChin

4、eseMedicine，Guangzhou510360，China；2．LaboratoryAnimalCenterofZhongshanUniversity，Guangzhou510080)【Abstract】ObjectiveTostudytheinhibitoryeffectofcurcuminonthegrowthofhumancervicalcarcinomaCaSkixenografisinnudemice．MethodsHumancervicalcancercelllineCaSkicellswereinocu

5、latedsubcutaneouslyintonudemice．Thirty—fivetumor—bearingmicewererandomlydividedinto5groups：curcuminhighdose(200mg／kg)，moderatedose(100mg／kg)，lowdose(50ms／kg)，solventcontrolgroupandcisplatingroup(3ms／kg)，7miceineachgroup．Cureuminwasorallyadministeredonceperdayforcon

6、secutive15days．Intraperitonealinjectionofcisplatinwasgiveneverythreedays，foratotaloffivetimes．Themiceweresacrificed24haftertheendoftreatmentandthebodyweight，tumorvolume，tumorweightweremeasuredandtheinhibitionratewascalculated．Thehistologicalchangesoftumortissuewase

7、xamined．ResultsThetumorvolumesofthetreatedgroupweresignificantlylowerthanthatofthecontrolgroup(P<0．01)．Thetumorinhibitionrateofthecurcuminhigh，mediumandlowdosegroupswere44．3％，40．1％and33．3％．respectively．Apparentnecrosiswasobservedinthetumorsofthecurcumin·treatedgrou

8、ps．ConclusionCurcuminpossessesapparentinhibitoryeffectonthegrowthoftransplantedtumorsofhumancervicalcancerCaSkicellsinnudemice．【Keywords】Cervical