資源描述：
《長裂苦苣菜水提取物對肺癌細(xì)胞A549增殖和凋亡的影響-論文.pdf》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、天然產(chǎn)物研究與開發(fā)NatProdRe*Dev2014，26：1380-1384文章編號：1001-6880(2014)9-1380-05長裂苦苣菜水提取物對肺癌細(xì)胞A549增殖和凋亡的影響賀燕云，劉小敏，張彩艷，華子義上海大學(xué)生命科學(xué)學(xué)院實(shí)驗(yàn)教學(xué)中心，上海2004．44-摘要：為了探討長裂苦苣菜水提取物對肺癌細(xì)胞A549的影響，本研究用不同濃度的長裂苦苣菜水提取物作用于體外培養(yǎng)的A549細(xì)胞，從細(xì)胞形態(tài)(顯微鏡觀察)、細(xì)胞增殖活力(CCK-8法)、細(xì)胞凋亡(AnnexinV-HTCJPI染色)、線粒體膜電位(jc-1染色)和細(xì)胞內(nèi)活性氧水平(熒光探針DC
2、FH-DA染色)幾個方面檢測長裂苦苣菜水提取物對體外培養(yǎng)的A549細(xì)胞的影響。當(dāng)處理濃度達(dá)到1mg／mL作用細(xì)胞48h后，細(xì)胞出現(xiàn)明顯皺縮的凋亡形態(tài)學(xué)特征，與對照組相比處理組細(xì)胞增殖活力明顯下降(P<0．05)。進(jìn)一步用流式細(xì)胞儀檢測發(fā)現(xiàn)≥1mg／mL的處理濃度作用細(xì)胞48h后細(xì)胞凋亡率顯著提高(P<0．05)，同時檢測到處理組細(xì)胞的膜電位都明顯下降，細(xì)胞內(nèi)活性氧水平明顯上升，都呈劑量依賴關(guān)系。這些結(jié)果表明長裂苦苣菜水提取物可以誘導(dǎo)A549細(xì)胞凋亡，并抑制其生長和增殖，是一種潛在的預(yù)防和抑制腫瘤生長的藥食兩用植物。關(guān)鍵詞：長裂苦苣菜；肺癌細(xì)胞；增殖；凋亡
3、中圖分類號：Q28文獻(xiàn)標(biāo)識碼：AApoptosisofLungCancerLineA549InducedbySonchusbranchyotusDCAqueousExtractsHEYan—yun，LIUXiao—ruing，ZHANGCai—yan，HUAZi—yiExperimentalCenterTorSciences，SchoolofSciences，ShanghaiUniversity，Shanghai200444，ChinaAbstract：ToillustratetheefectsofSo淞branchyotusDC(SBDC)aqueou
4、sextractsonhumanlungcancerA549cells，assays，includingmorphologiechangesobservedbymicroscope，cellproliferationvitalitytestedbycelgrowthassayusingcck8kit，theapoptosisdetectedbyHowCytometry，mitochondrialmembranepotentialasses8edwiththefluorescentprobeJC-1andreactiveoxygenspeciesforma
5、tionmeasuredbyDCFH-DAstaining，wereperformed．Observedwithmicro-scope，cellstreatedwithhishdose(I>1m#mL)ofSBDCwereshrunkenandthemorphologicalcharacteristicsofapop-tosiswasobviouscomparedwiththecontrolgroup．Also，cellproliferationWagsignificantlyinhibitedcomparedwiththecontrolgroup(P<
6、0．05)．Furthermore，floweytometryanalysisrevealedtheapoptesiscellstreatedwithhishdoseofSB—DC(~>lmg／mL)for48hoursweresignificantlyincreasedcomparedwiththecontrolgroup．Finally。it嗍foundinalltreatmentgroupsthatthesignificantdecreaseofcelmitochondrialmembranepotentialandtheincreaseofrea
7、ctiveoxy-genspeciesleve1．TheseresultsdemonstratedthatSBDCmayinducehumanlungcancerA549cellsapoptesisandinhibittheceilsgrowthandproliferation．SBDCisapotentialedibleherbforcancerpreventionandtreatment．Keywords：SonchusbranchyotusDC；lungcancercel；apoptosis；proliferation長裂苦苣菜(Sonchusbr
8、achyotusDC．)是菊科植保健作用較為明顯。文獻(xiàn)報(bào)道苦苣菜具有消炎、抗物苦
