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1、老年醫(yī)學(xué)與保健2013年第l9卷第6期GeriatrHealthCare,2013,Vo1.19.No.6●論著●淫羊藿苷延長秀麗線蟲壽命與機(jī)制吳俊珍夏世金孫濤蔡外嬌黃建華寧友吳金峰彭正羽【摘要】目的探討淫羊藿苷(Icariin,Ica)對(duì)秀麗線蟲壽命的干預(yù)效果與機(jī)制,為篩選延緩衰老、延長壽命的藥物提供實(shí)驗(yàn)依據(jù)。方法將秀麗線蟲分為空白對(duì)照組、Ica5mg/L、10mg/L、15mg/L、2Omg/L各劑量干預(yù)實(shí)驗(yàn)組,觀察在Ica不同梯度劑量作用下秀麗線蟲壽命的變化,以明確Ica作用的最佳劑量。接著設(shè)立秀麗線蟲空白對(duì)照組和
2、Ica(15mg/L)干預(yù)組,評(píng)價(jià)Ica對(duì)秀麗線蟲平均壽命、生殖能力的干預(yù)效果;同時(shí)進(jìn)行急性熱應(yīng)激和急性氧化應(yīng)激實(shí)驗(yàn),觀察應(yīng)激時(shí)線蟲的平均生存時(shí)間,探討Ica延長線蟲壽命的機(jī)制。結(jié)果15mg/L的Ica是延長秀麗線蟲壽命的最佳劑量。Ica組線蟲的平均壽命明顯長于空白對(duì)照組【(31.87±4.46)dvs.(20.13土2.81)d】(P<0.O1);Ica組線蟲生殖高峰期子代數(shù)目比空白對(duì)照組顯著增加(151+21.13v8.122±17.09,P
3、明顯長于空白對(duì)照組【(9.53±1.34)hvs.(5.97+0.84)h,(8.34士1.17)hvs.(5.77士O.81)hi(P<0.01o結(jié)論淫羊藿苷能延長秀麗線蟲的壽命,其機(jī)制可能與淫羊藿苷提高線蟲的應(yīng)激能力有關(guān)。[關(guān)鍵詞】壽命;衰老;秀麗線蟲;淫羊藿苷;熱應(yīng)激;氧化應(yīng)激;生殖能力【中圖分類號(hào)】R339.8[文獻(xiàn)標(biāo)識(shí)碼】A[文章編號(hào)】1008—8296(2013)一06—349—05D0I:10.3969~.issn.1008—8296.2013—06EffectoficariinoillifespanofC
4、aenorhabditiseleganswUJun—zhen.X1AShi-jin*,SUNTao.CAIWai-jiao.HUANGJian—hua,N1NGYou.wJin-feng,PENGZheng-yu.DepartmentofGeriatrics,HuadongHospital,FudanUniversity,Shanghai200040,ChinaCorrespondingauthor:XIAShi-jin,E—mail:xiashijinhd@163.comIAbstract】ObjectiveToeva
5、luatetheeffectofIcariin(Ica)onlifespanofCelegansinordertoscreendrugsforlongevity.Methods(1)Dosage—effecttest:eelegans,whichweretreatedwithIca0mg/L,Ica5mg/L,10mg/L,15mg/L,2Omg/L,respectively,weretestedbylifespanassaytodeterminethebesteffectivedosagesofIca.(2)Lifes
6、panassay:C.elegansweredividedintocontrolgroupandIca(15mg/L)treatmentgroup.Lifespanassaywasusedtoevaluatethelife-extensioneffectofIcaonCelegans.(3)Fecunditytest:eelegansfecundityintheIca(15mg/L)treatmentandcontrolgroupswasassessedbyevaluatingtheeffectofIcaonthewor
7、ms’reproduction.(4)Stressresistancetest:thelifespanofeelegansintheIca(15mg/L)treatmentandcontrolgroupswereassessedbyacuteheatandoxidativestresstreatmenttoexploretheinitialmechanismsinvolvedintheeffectofIcaonlifespan.Results15mg/LofIcawasthebestdosagetomadeeelegan
8、sgetthemaximumlifespan.TheaveragelifespanofC.elegansintheIca·treatedgroupwassig—nificantlylongerthanthecontrolgroup【(31.87±4.46)dvs.(20.13±2.81)d】(P