分離膠的配方.doc

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1、1.分離膠的配方溶液成分不同體積（ml）凝膠液中各成分所需體積（ml）5101520253040506%????????水2.65.37.910.613.215.921.226.530%丙烯酰胺溶液1.02.034568101.5mol/LTris(pH8.8)1.32.53.856.37.51012.510%SDS0.050.10.150.20.250.30.40.510%過硫酸氨0.050.10.150.20.250.30.40.5TEMED0.0040.0080.0120.0160.020.0240.0320.048%????????水2.34.66.99.311.513.9

2、18.523.230%丙烯酰胺溶液1.32.745.36.7810.713.31.5mol/LTris(pH8.8)1.32.53.856.37.51012.510%SDS0.050.10.150.20.250.30.40.510%過硫酸氨0.050.10.150.20.250.30.40.5TEMED0.0030.0060.0090.0120.0150.0180.0240.035??10?1520??25?30405010%水1.945.97.99.911.915.919.830%丙烯酰胺溶液1.73.356.78.31013.316.71.5mol/LTris(pH8.8)1.

3、32.53.856.37.51012.510%SDS0.050.10.150.20.250.30.40.510%過硫酸氨0.050.10.150.20.250.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.0212%????????水1.63.34.96.68.29.913.216.530%丙烯酰胺溶液2468101216201.5mol/LTris(pH8.8)1.32.53.856.37.51012.510%SDS0.050.10.150.20.250.30.40.510%過硫酸氨0.050.10.150.20.250.30.4

4、0.5TEMED0.0020.0040.0060.0080.010.0120.0160.0215%????????水1.12.33.44.65.76.99.211.530%丙烯酰胺溶液2.557.51012.51520251.5mol/LTris(pH8.8)1.32.53.856.37.51012.510%SDS0.050.10.150.20.250.30.40.510%過硫酸氨0.050.10.150.20.250.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.022.配制Tris-甘氨酸SDS-PAGE聚丙烯酰胺凝膠電泳5%積

5、層膠（濃縮膠）所用溶液溶液成分不同體積（ml）凝膠液中各成分所需體積（ml）123456810?????????水0.681.42.12.73.44.15.56.830%丙烯酰胺溶液0.170.330.50.670.8311.31.71.0mol/LTris(pH6.8)0.130.250.380.50.630.7501.2510%SDS0.010.020.030.040.050.060.080.110%過硫酸氨0.010.020.030.040.050.060.080.1TEMED0.0010.0020.0030.0040.0050.0060.0080.01