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1、宜賓職業(yè)技術(shù)學(xué)院論文α-淀粉酶的生產(chǎn)工藝系部生物與化工工程系專(zhuān)業(yè)名稱(chēng)釀酒技術(shù)班級(jí)釀酒11103姓名王夢(mèng)韻李艷梅嚴(yán)紅俊學(xué)號(hào)201117315201117404201117346指導(dǎo)教師蘭小艷二〇一二年十月二十九日α-淀粉酶的生產(chǎn)工藝摘要α-淀粉酶廣泛分布于動(dòng)物、植物和微生物中，能水解淀粉產(chǎn)生糊精、麥芽糖、低聚糖和葡萄糖等，是工業(yè)生產(chǎn)中應(yīng)用最為廣泛的酶制劑之一。目前，α-淀粉酶已廣泛應(yīng)用于變性淀粉及淀粉糖、焙烤工業(yè)、啤酒釀造、酒精工業(yè)、發(fā)酵以及紡織等許多行業(yè)。本次設(shè)計(jì)的淀粉酶發(fā)酵，分別以玉米粉為碳源，以豆餅
2、為氮源，以BF-7658枯草芽孢桿菌為生產(chǎn)菌種,同時(shí)做出了生產(chǎn)工藝流程圖，詳細(xì)的介紹了α-淀粉酶的生產(chǎn)工藝。關(guān)鍵詞：α-淀粉酶；生產(chǎn)工藝設(shè)計(jì)；深層發(fā)酵法AlphaamylaseproductiontechnologyAbstractAlphaamylasewidelydistributedinanimals,plantsandmicrobes,canbehydrolyzedstarchproducedextrin,maltose,oligosaccharideandglucoseandsoon,itis
3、theindustrialproductioninthemostwidelyusedoneofenzymepreparation.Atpresent,thealphaamylasehasbeenwidelyusedinmodifiedstarchandstarchsugar,bakingindustry,brewing,alcoholindustry,hairleavenandtextileandmanyotherindustries.Thedesignofamylasefermentation,res
4、pectivelywithcornflourforcarbonsource,nitrogensourceforbeancake,withBF-7658bacillussubtilisstrainsfortheproduction,atthesametime,maketheproductionprocessflowdiagram,detailedintroducesthealphaamylaseproductiontechnology.Keywords:Alphaamylase,Productionpro
5、cessdesign;Deepfermentation目錄前言................................................................................................................................11α-淀粉酶的生產(chǎn)方法...................................................................................
6、...............11.1生產(chǎn)方法的選擇...................................................................................................11.2α-淀粉酶培養(yǎng)基的制作....................................................................31.2.1耐高溫α-淀粉酶的生產(chǎn)方法...........................
7、.................................................41.2.2耐高溫α－淀粉酶的生產(chǎn)原料.........................................................................41.2.3耐高溫α－淀粉酶培養(yǎng)基制作.........................................................................51.2.4耐高溫α－淀粉酶的另一種生產(chǎn)方
8、法.............................................................51.2.5用菌種枯草芽孢桿菌來(lái)提取耐高溫α－淀粉酶.............................................52α-淀粉酶的菌種選育......................................................................62.1菌株與
