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《基于10個(gè)棉花腺體相關(guān)材料轉(zhuǎn)錄組的EST-SSR標(biāo)記開(kāi)發(fā)》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、棉花學(xué)報(bào)CottonScience2015袁27淵1冤院1―8基于10個(gè)棉花腺體相關(guān)材料轉(zhuǎn)錄組的EST-SSR標(biāo)記開(kāi)發(fā)*程海亮袁陸才瑞袁鄒長(zhǎng)松袁余道乾袁姜鵬飛袁楊文翠袁張友平袁王巧連袁宋國(guó)立淵中國(guó)農(nóng)業(yè)科學(xué)院棉花研究所/棉花生物學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室袁河南安陽(yáng)455000冤摘要院棉花是重要的經(jīng)濟(jì)作物袁但有毒棉酚的存在袁使棉子得不到充分的利用袁所以低酚棉育種是棉花育種的重要內(nèi)容之一遙色素腺體是棉酚的儲(chǔ)存器官袁開(kāi)發(fā)功能型分子標(biāo)記袁加密棉花遺傳圖譜對(duì)色素腺體和其他重要性狀相關(guān)基因的研究具有重要作用遙本試驗(yàn)以10個(gè)有腺體和無(wú)腺體材料轉(zhuǎn)錄組信息為基礎(chǔ)開(kāi)發(fā)EST-SSR標(biāo)記袁在12895條1kb以上的Unigen
2、e中共搜索到1546個(gè)SSR位點(diǎn)袁發(fā)生頻率和平均距離分別是11.99%和15.99kb遙得到的EST-SSR中袁二堿基和三堿基重復(fù)是主要的重復(fù)類型袁分別占30.85%和48.97%袁AT/TA和GAA/CTT分別是二堿基和三堿基的優(yōu)勢(shì)重復(fù)類型遙對(duì)長(zhǎng)度逸20bp的SSR共設(shè)計(jì)合成了56對(duì)引物袁在這10個(gè)材料中進(jìn)行檢測(cè)袁能擴(kuò)增出清晰條帶的有38對(duì)袁占67.86%袁其中呈多態(tài)性的有9對(duì)袁占23.68%袁并對(duì)其所在的Unigene功能和表達(dá)量進(jìn)行了初步分析遙本研究進(jìn)一步證明了棉花EST-SSR標(biāo)記開(kāi)發(fā)的可行性袁并且為棉花高密度遺傳圖譜和腺體相關(guān)基因的研究奠定了基礎(chǔ)遙關(guān)鍵詞院棉花曰EST-SSR標(biāo)記曰多
3、態(tài)性曰色素腺體中圖分類號(hào)院S562.035.3文獻(xiàn)標(biāo)志碼院A文章編號(hào)院1002-7807淵2015冤01-0001-08ChengHailiang,LuCairui,ZouChangsong,YuDaoqian,JiangPengfei,YangWencui,ZhangYouping,*WangQiaolian,SongGuoli(455000,)Cottonisanimportanteconomiccrop,however,thecottonseedisunderutilizedbecauseofthepresenceoftoxicgossy-pol,solowgossypolcottonb
4、reedingisanimportantpartofthecottonbreeding.Thedevelopmentofmolecularmarkersandconstructionofhighresolutiongeneticmapwillplayanimportantroleinresearchofgenesrelativetopigmentglands,whicharestorageorganofgossypol,andotherimportanttraits.Inthisstudy,wedevelopedEST-SSRmarkersbasedonthetranscriptomeinfo
5、rmationfrom10glandandglandlessmaterials.1546EST-SSRswereobtainedfrom12895unigenesmorethan1kbinlength,whichaccountsfor11.99%,andtheaveragedistanceofSSRswas15.99kb.AmongtheobtainedEST-SSRs,dinu-cleotideandtrinucleotiderepeatswerethedominanttypes,accountingfor30.85%and48.97%respectively.AT/TAandGAA/CTT
6、werethemostabundantmotifsrespectivelyindinucleotideandtrinucleotiderepeats.Totalof56pairsofprimersweredesignedandsynthesizedfortheSSRsmorethan20bpandtestedinthe10materialstoevaluatetheapplicationandpoly-morphism.38pairsofprimerscouldproduceclearbandsinPAGEelectrophoresis,accountingfor67.86%,and9EST-
7、SSRsa-mongthemshowedpolymorphism,accountingfor23.68%.Furthermore,thefunctionsandexpressionoftheunigenescontain-ingpolymorphicEST-SSRwereanalyzedpreliminarily.Thisstudyfurtherprovesthatitisfeasibletode
