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1、學(xué)校代碼:10663學(xué)號:15010100556貴州師范大學(xué)碩士學(xué)位論文青籬柴轉(zhuǎn)錄組高通量測序分析及SSR分子標記的開發(fā)High-throughputsequencingAnalysisanddevelopmentofSSRmolecularmarkersinthetranscriptomeofTirpitziasinensis專業(yè)名稱:生態(tài)學(xué)專業(yè)代碼:071300研究方向:植物繁殖生態(tài)學(xué)申請人姓名:黃建敏導(dǎo)師姓名:湯曉辛二零一八年六月目錄摘要..........................................................
2、IAbstract......................................................III第一章文獻綜述..............................................11.1青籬柴及其研究進展....................................11.2高通量轉(zhuǎn)錄組測序相關(guān)背景介紹..........................11.2.1轉(zhuǎn)錄組定義.......................................11.2.2測序技術(shù)的發(fā)展.......
3、............................21.2.3轉(zhuǎn)錄組測序.......................................31.3遺傳標記研究進展......................................41.3.1遺傳標記.........................................41.3.2SSR標記研究進展.................................51.4研究背景、目的及意義..................................61.4.1研
4、究背景.........................................61.4.2研究目的和意義...................................6第二章青籬柴轉(zhuǎn)錄組生物信息學(xué)分析............................82.1研究材料..............................................82.1.1植物材料.........................................82.1.2實驗試劑..............................
5、...........82.1.3實驗儀器.........................................82.2研究方法..............................................82.2.1青籬柴總RNA提取.................................82.2.2總RNA檢測.......................................82.2.3測序文庫構(gòu)建.....................................92.2.4轉(zhuǎn)錄組測序.......
6、................................92.2.5轉(zhuǎn)錄組數(shù)據(jù)的組裝.................................92.2.6轉(zhuǎn)錄組數(shù)據(jù)的分析.................................92.3結(jié)果與分析...........................................102.3.1RNA提取及質(zhì)量檢測..............................102.3.2轉(zhuǎn)錄組測序與組裝................................112.3.3轉(zhuǎn)錄組
7、序列的功能注釋............................122.3.4KOG分類........................................132.3.5GO分類.........................................142.3.6KEGG代謝通路分析...............................172.3.7編碼蛋白框(CDS)預(yù)測...........................242.3.8基因轉(zhuǎn)錄因子分析................................27
8、2.3.9基因表達水平分析.............