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1、·150·中國(guó)疼痛醫(yī)學(xué)雜志ChineseJournalofPainMedicine2014，20(3doi：10．3969~．issn．1006-9852．2014．03．006神經(jīng)病理性疼痛脊髓背角GLT-1及GLAST表達(dá)的變化羅裕輝張德仁(廣東醫(yī)學(xué)院附屬南山醫(yī)院，深圳518052)摘要目的：觀察神經(jīng)病理性疼痛大鼠脊髓背角GLT-1及GLAST表達(dá)變化對(duì)疼痛的影響。方法：選取雄性SD大鼠80只隨機(jī)分為4組，A組=10)為k背根切斷假手術(shù)組，B組(7z=30)為k背根切斷組，c組(7z=10)為坐骨神經(jīng)周圍植管與藥
2、物溶劑組，D組=30)為坐骨神經(jīng)周圍植管與給藥組。通過(guò)機(jī)械刺激的行為學(xué)觀察確立病理性神經(jīng)痛形成過(guò)程，采用免疫組化進(jìn)行細(xì)胞定量。結(jié)果：A及C組膠質(zhì)細(xì)胞GLT-1及GLAST表達(dá)無(wú)變化。B、D組痛敏形成后脊髓背角膠質(zhì)細(xì)胞GLT-1及GLAST的胞內(nèi)外表達(dá)未發(fā)生改變；B及D組小膠質(zhì)細(xì)胞GLAST術(shù)后4d表達(dá)增加至7d后逐漸下降，而星型膠質(zhì)細(xì)胞GLT-1術(shù)后6d表達(dá)明顯增加，直至14d仍持續(xù)增高。結(jié)論：脊髓背角膠質(zhì)細(xì)胞GLT-1及GLAST不存在胞吞現(xiàn)象，小膠質(zhì)細(xì)胞參與啟動(dòng)病理性神經(jīng)痛但不維持其發(fā)展，星型膠質(zhì)細(xì)胞主要維持病理
3、性神經(jīng)痛發(fā)展，TNF．儀介導(dǎo)病理性神經(jīng)痛大鼠脊髓背角GLT-1及GLAST表達(dá)并誘導(dǎo)膠質(zhì)細(xì)胞參與和維持病理性神經(jīng)痛。關(guān)鍵詞神經(jīng)病理性疼痛；腫瘤壞死因子．；脊髓背角；谷氨酸轉(zhuǎn)運(yùn)體EXPRESSIONOFGLT．1ANDGLASTINSPINALCORDDORSALHoRNWITHNEURoP．ATHICPAINLUOYu．Hui，ZHANGDe．Ren△(AfiliatedNanshanHospitalofGuangdongMedicalCollege，Shenzhen518052)Abstract0bjective：
4、ToobservetheefectoftheexpressionofGLT-1andGLASTinspinalcorddorsalcordwithneuropathicpain．Methods：EightymaleSDratswererandomlydividedintofourgroups．GroupA=10)wastreatedwithshalTloperationwithL5dorsalroottransection，groupB(7z=30)underwentL5dorsalroottransection，g
5、roupC=10)receivedintubationandmedicinesolventectionaroundthesciaticnerveandgroupDreceivedintubationaroundthesciaticnerveandTNF一administration．Mechanicalstimulationwasadaptedtoestablishtheformationprocessofneuropathicpainandimmunohistochemistrywasappliedtoquanti
6、fytherelatedsubstancesandcells．Results：NochangesappearedintheexpressionofGLT-1andGLASTingroupsBandDafterneuropathicpainWasestablished．TheexpressionofGLT-1inrnicroglialcellsincreasedat6claysafteroperation，andcontinuedtoincreasethrough14daysingroupsBandD．GLASTofa
7、strocytesshowedincreasedexpressionat4claysafteroperationuntilthe7clayswhentheexpressionbegantodecreasegradually．TherewerenochangesofGLT-IandGLASTintheglialcellsingroupsAandC．Conclusion：ThereisnoendocytosisphenomenonofGLT-1andGLASTinglialcells．Themicroglialcells
8、areinvolvedintheinductionofneuropathicpainbutdonotsustainthepaindevelopment，andtheastrocytesplayaroleinthesustainmentofpain．TNF—O／．mediatestheexpressionofGLT-1andGLASTinspin
