17β-雌二醇對(duì)骨髓基質(zhì)細(xì)胞成骨、成脂分化和成骨細(xì)胞成脂橫向分化的影響

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1、17β-雌二醇對(duì)骨髓基質(zhì)細(xì)胞成骨、成脂分化和成骨細(xì)胞成脂橫向分化的影響17/I.雌二醇對(duì)骨髓基質(zhì)細(xì)胞成骨,成脂分化和成骨細(xì)胞成脂橫向分化的影響李湘輝-.,張金超,-,劉長(zhǎng)振,隋森芳,楊夢(mèng)延2,3(1.清華大學(xué)生物科學(xué)與技術(shù)系,生物膜國(guó)家重點(diǎn)實(shí)驗(yàn)室,北京100084;2香港城市大學(xué)深圳研究院,深圳518057;3.香港城市大學(xué)生物及化學(xué)系,香港;4.河北大學(xué)化學(xué)與環(huán)境科學(xué)學(xué)院,河北保定071002)摘要:目的研究17fl一雌二醇對(duì)小鼠原代骨髓基質(zhì)細(xì)胞成骨,成脂分化以及小鼠原代成骨細(xì)胞成脂橫向分化的影響.方法四甲基偶氮唑鹽法(1帆')檢測(cè)17fl一雌二醇對(duì)骨髓基質(zhì)細(xì)胞和成骨細(xì)

2、胞增殖的影響,堿性磷酸酶(AIP)活性測(cè)定法檢測(cè)17fl一雌二醇對(duì)骨髓基質(zhì)細(xì)胞成骨分化的影響,油紅.染色形態(tài)學(xué)及定量測(cè)定法檢測(cè)17f1.雌二醇對(duì)骨髓基質(zhì)細(xì)胞成脂分化和成骨細(xì)胞成脂橫向分化的影響.結(jié)果1×10一,1×10..,5×10～,1×10'..,5×10一和1×10一mol-L一的17一雌二醇可以促進(jìn)小鼠原代骨髓基質(zhì)細(xì)胞和成骨細(xì)胞增殖(P<0.05);5×10..,1×10一,5×10和1×10mol?L-1的17一雌二醇促進(jìn)骨髓基質(zhì)細(xì)胞的成骨分化,抑制成脂分化,但是濃度為1×10mol-L-1的17.雌二醇抑制骨髓基質(zhì)細(xì)胞的成骨分化,促進(jìn)成脂分化(P<

3、0.05);濃度為1×10一,5×10..,1×10...,和1×10mol?L-1的17.雌二醇抑制成骨細(xì)胞的成脂分化(P<0.05).結(jié)論17f1.雌二醇可以調(diào)節(jié)骨髓基質(zhì)細(xì)胞向成骨細(xì)胞分化而減少其向脂肪細(xì)胞分化,并且可以抑制成骨細(xì)胞向脂肪細(xì)胞的橫向分化.關(guān)鍵詞:17f1.雌二醇;骨髓基質(zhì)細(xì)胞;成骨細(xì)胞;成骨分化;成脂分化;成脂橫向分化中圖分類號(hào):R965文獻(xiàn)標(biāo)識(shí)碼:A文章編號(hào):1001—2494(2o06)17—1305—05Effectsof17/I-estradioionOsteogenicandAdipogenicAdipocyticTrans-Diffe

4、rentiationofOsteoblastsLIXiang.hui,,ZHANGJin.chao3,,LIUChang-zhen,SUISen-fang,YANGMeng-SU2,.(1.StateKeyLaboratoryofBiornembrane,Departmentof8/olog~Sc/encesandBio~hnology,TsinghuaUnlversity,&100084,Ch/na;2.ShenzhenReaserchInstitute,CityUniversityofHongKong,Shenzhen518057,Ch/na;3.Oepanm

5、~ofBiologyandChemistry,CityUniversityofHongKong,HongKong.Ch/na;4.Oepanm~ofChemistry,CollegeofChemistry&EnvironmentalScien~,HebeiUniversity,Baoding071002,Ch/一/la)ABSIRACT:OBJECTIVETostudytheeffectsof17?estradiolontheosteogenicandadipogenicdifferentiationofprimarymOll~bonemarrowstromalc

6、ells(MSCs)andtheadipocytictrans-differentiationofprimarylnouseosteoblasts(OBs).MEn3?(4,5一Dimethylthia?zd-2-y1).2,5一diphenyltetrazoliumbromide(MTF)wasusedtostudytheeffectsof17?estradiolonthegrowthofMsCsandOBs,alkalinephosphatase(MY)activityassaywasusedtostudytheeffectsof17—estradiolonosteo

7、genicdifferentiationofMsCs,andOilRedOassaywasusedtostudytheeffectsof17口一estradiolontheadipogenicdifferentiati0n0fMScsandadipocytictran~differentiati0nofOBs.REsI】I17J9一estradiolpromoted出eproliferationofprimary玎10usebonefTlarrDw$trol~cellsandimarym0useosteoblaslsatthe