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1、uPA和uPARmRNA高表達與肝細胞癌的關系?394?生第l3卷第5期ChinJClinOneolRehabil.Oct0ber2006.V0113.N0.5uPA和uPARmRNA高表達與肝細胞癌的關系?臨床研究?周奇張亮胡瓊珍張福君甄宇洋(1.中山大學第一附屬醫(yī)院黃埔院區(qū)外一科,廣州510700;2.中山大學附屬腫瘤醫(yī)院影像介入中心,華南腫瘤學國家重點實驗室;3.南方醫(yī)科大學珠江醫(yī)院層流病房)【摘要】目的檢測uPA,uPAR在肝癌組織中的表達并探討其臨床意義.方法用RT—PCR法對肝癌,癌旁,正常組織各27例進
2、行uPA,uPAR檢測,并結(jié)合臨床病理資料進行分析.結(jié)果uPA,uPAR在肝癌組織中陽性率分別為70.4%(19/27)和77.8%(21/27);相對表達強度分別為0.964±0.458和0.784±0.322,均較癌旁組織及正常組織高,差異有顯著性.陽性患者相對表達強度與腫瘤大小及部分侵襲轉(zhuǎn)移指標中表達呈顯著相關.結(jié)論uPA,uPAR在肝癌組織及部分侵襲轉(zhuǎn)移指標中表達升高,提示二者可能參與了肝細胞癌的發(fā)生及侵襲轉(zhuǎn)移.【關鍵詞】肝腫瘤;uPA;uPAR【中圖分類號】R735.7;R730.43【文獻標識碼】A【文章
3、編號】1005.8664(2006)05-0394-04OverexpressionofuPAanduPARmRNAintissueofhepatocellularcarcinomaanditsclinicalsignifi-CauceZHOUQi,ZHANGLiang,HUQiong—zhen.etal(The1stSurgeryDepartmentofHuangpuBranchoftheFirstAffiliatedHospital,ZhongshanUniversity,Guangzhou510700,Chin
4、a)【Abstract】0bjectiveTodetectthemRNAexpressionofuPAanduPARinsamplesofHCCandadjacenttissueandtoelucidateitsclinicalsignificance.MethodsTwenty—sevenhumanhepatocellularcar-cinomatissuesampleswiththeiradjacenttissuesamplesand27normallivertissuesampleswereexaminedby
5、reversetranscription—polymerasechainraction(RT—PCR).TherelationshipbetweenthemRNAexpressionandtheirchnic0path0l0caldatawereanalyzed.ResultsTheexpressionratesofuPAanduPARinhepato—cellularcarcinomatissuesampleswere70.4%(19/27)and77.8%(21/27)respectivelyandtherela
6、tiveex—pressionintensityofuPAanduPARwas0.964±0.458and0.784±0.322respectively.Theyweresignifi—candyhigherthanthoseintheadjacenttissuesamplesand27normallivertissuesamples(P<0.05).Therelativeexpressionintensityofu—PAandu—PARwerealsosignificantlyhigherinthesubgr
7、oupwithcarcino—masoflargersizeandseveralinvasiveandmetastaticindexes(P<0.05).ConclusionsExpressionofuPAanduPARmRNAlevelswassignificantlyhigherinhepatocellularcarcinomatissuesamplesandinthesub—groupwithinvasiveandmetastaticindexesthaninadjacenttissuesamplesan
8、dnormallivertissuesamples,indicatingthattheymightplayimportantrolesinhepaticcarcinogenesis,invasivenessandmetastasis.【Keywords】Hepaticneoplasms;uPA;uPAR腫瘤細胞水解細胞外基質(zhì)是腫瘤浸潤和轉(zhuǎn)移過程
