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1、1降低神經(jīng)病理性疼痛大鼠海馬和PAGIL-6mRNA的表達(dá)第44卷第10期2006年10月山東大學(xué)(醫(yī)學(xué)版)JOURNALOFSHANDONGUNWERSffY(HEALTHSCIENCES)V01.44No.100et.20o6文章編號(hào):1671—7554(2006)10—1028一o4側(cè)腦室注射內(nèi)嗎啡肽一1降低神經(jīng)病理性疼痛大鼠海馬和PAGIL一6mRNA的表達(dá)孫濤,宋文閣,姚尚龍,傅志儉(1.山東省立醫(yī)院疼痛科,山東濟(jì)南250021;2.華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬協(xié)和醫(yī)院麻醉科,湖北武漢430022)[摘要]目的:觀察側(cè)腦室微量注射內(nèi)嗎啡肽.1(endomorphin.1,EM.1
2、)對(duì)坐骨神經(jīng)慢性壓迫性損傷(chronicconstrictioninjury,CCI)大鼠痛閾以及海馬和中腦導(dǎo)水管周圍灰質(zhì)(periaqueductalgray,PAG)中白細(xì)胞介素.6(interleukin.6,IL-6)mRNA表達(dá)的影響.方法:建立大鼠慢性坐骨神經(jīng)壓迫性損傷模型,側(cè)腦室微量注射EM.1(20g/20),手術(shù)前1d和手術(shù)后第7d觀察其機(jī)械性痛敏和熱痛敏的變化,術(shù)后第7d將大鼠處死,采用原位雜交觀察海馬和PAGIL-6mRNA的表達(dá),假手術(shù)及側(cè)腦室注射生理鹽水作對(duì)照.結(jié)果:實(shí)驗(yàn)發(fā)現(xiàn)CCI手術(shù)導(dǎo)致大鼠機(jī)械性痛閾和熱痛閾明顯下降,且增加大鼠海馬和PAG部位IL-6mRN
3、A的表達(dá).側(cè)腦室注射EM.1可提高大鼠基礎(chǔ)痛閾,且顯著改善大鼠神經(jīng)病理性疼痛狀態(tài),降低海馬和PAG部位IL-6mRNA的表達(dá).結(jié)論:側(cè)腦室微量注射EM.1可有效抑制神經(jīng)病理性疼痛,且明顯降低海馬和PAGIL-6的表達(dá).[關(guān)鍵詞]慢性壓迫性損傷;內(nèi)嗎啡肽.1;白細(xì)胞介素6[中圖分類號(hào)]R614[文獻(xiàn)標(biāo)識(shí)碼]ADown?-regulationofinterleukin?-6mRNAinhippocampusandofPAGinchronicneuropathicpainmodelofratsafterintracerebroventricularmicroinjectionofendomor
4、phin一1SUNTao,SONGWen.ge,YAOShang.1ong2,FUZhi.jian(1.DepartmentofPainManagement,ShandongProvincialHospital,Jinan250021,Shandong,China;2.DepartmentofAnesthesiology,UnionHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430022,Hubei,China)[ABSTRACT]Objective:Toinvestigateth
5、eeffectofintracerebroventricular(i.c.v.)injectionofendomorphin-1(EM-1)onIL-6mRNAexpressionlevelinhippocampusandonPAGinchronicconstrictioninjury(CCI)modelofrats.Methods:TheCCImodelwasestablishedandgivenendomorphin-1(20∥20,i.C.v)oncedailyfor3consec-utivedays.ThecontrolratswereoperatedonandinjectedN
6、S.Themechanicalandthethermalpainthresholdweremen-sured1daybeforeoperationand7daysafteroperation.TheexpressionlevelofIL-6mRNAWasmeasuredbyinsituhy-bridization.Results:MechanicalallodyniaandthermalhyperalgesiawereinducedinCCIrats.Inaddition,theexpres-sionlevelofIL-6mRNAinhippocampusandPAGmarkedlyin
7、creased.IntracerebmventricularinjectionofEM-1in-creasedthebasicpainthresholdoftherats,reversedtheCCI-inducedexistingneuropathicpainstateandsignificantlysuppressedtheincreasedexpressionlevelofIL-6mRNAinducedbyCCI.Conclu
