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1、授予單位代碼10089學(xué)號或申請?zhí)?6215中國圖書分類號R68HebeiMedicalUniversity碩士學(xué)位論文學(xué)術(shù)學(xué)位新型復(fù)合支架對骨髓間充質(zhì)干細胞增殖及分化影響的研究研究生：王彥鵬導(dǎo)師：孫明林主任醫(yī)師專業(yè)：外科學(xué)二級學(xué)院：武警后勤學(xué)院附屬醫(yī)院2018年3月目錄中文摘要·······································································································1英文摘要····························
2、···········································································4英文縮寫·······································································································8研究論文新型復(fù)合支架對骨髓間充質(zhì)干細胞增殖及分化影響的研究前言·····························································
3、··········································9材料與方法····························································································13結(jié)果·······································································································21附圖·························
4、··············································································24討論·······································································································29結(jié)論·······················································································
5、················34參考文獻····························································································34綜述仿生支架修復(fù)骨組織研究進展·························································40致謝····················································································
6、······························50個人簡歷·······································································································51新型復(fù)合支架對骨髓間充質(zhì)干細胞增殖及分化影響的研究摘要目的：創(chuàng)傷、退行性病變或腫瘤等原因?qū)е碌墓侨睋p仍然是骨骼修復(fù)重建的主要難題。同種異體骨移植和自體骨移植是當(dāng)前治療骨缺損的最佳手段。而這兩種治療都有其局限性，如供體組織有限、供體部位發(fā)病率高、手術(shù)復(fù)雜、同種異體移植物疾
7、病傳播或免疫排斥的可能性、且移植物無法準(zhǔn)確塑形替代原組織的生物力學(xué)特性。如何實現(xiàn)骨缺損修復(fù)重建一直是當(dāng)今醫(yī)學(xué)界的難題，組織工程支架為解決這一難題提供了很好的思路。本實驗旨在采用冷凍干燥法制備膠原蛋白/殼聚糖/羥基磷灰石三維仿生骨組織工程支架，并觀察膠原蛋白/殼聚糖/羥基磷灰石三維復(fù)合支架對骨髓間充質(zhì)干細胞的增殖和分化的影響。目的是研究組織工程支架治療骨缺損修復(fù)重建的可行性。方法：1.大鼠骨髓間充質(zhì)干細胞（BMSCs）的分離培養(yǎng)和分化鑒定：抽取清潔級健康雄性SD大鼠骨髓，密度梯度離心法提取單個核細胞，貼壁法分離骨髓間充質(zhì)干細胞。通過CCK-8法檢測
8、細胞生長曲線；成骨誘導(dǎo)分化培養(yǎng)后，進行茜素紅染色檢測礦物結(jié)節(jié)染色評估成骨分化情況。2.膠原/殼聚糖/羥基磷灰石復(fù)合支架制備：采用冷凍干燥
