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《三聚氰胺的遺傳毒效應(yīng)及牛奶中三聚氰胺快速檢測方法研究》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、melaminefromdispersiontoaggregationrapidly,alongwiththecolorchangefromredtoblue(orpurple).Aftertheoptimizationofreactionconditions,thewholeanalysisonlyneedsabout20minincludingthesimplepretreatmentwedesigned.Theanalysiscanbeobservedbythenakedeye,andsi
2、mpletooperatewithoutsophisticatedinstruments.Intherawmilk,thepresentlimitofdetectionformelamineis1μMbynakedeyesanddownto375nMbytheinstument.Furthermore,weexploretheinteractionprinciplebetweenmelamineandcitrate-stabilizedAuNPsinthiswork;establishthefo
3、undationformodifyingtheAuNPsandoptimizingthesystemofAuNPs-melamineinthefuture.Inthispaper,wealsoreportarapidandsensitivecolorimetricsensorformelaminedetectioninmilkbytheaptamerbasednanoparticles(AuNPs).AptamercanabsorbonthesurfaceofAuNPsandelectrosta
4、ticallyinteractswithPoly(diallyldimethylammoniumchloride)(PDDA),whichprotectsAuNPsfromaggregating.Theintroductionofmelaminewouldformamelamine-aptamercomplexbyhydrogenbonds,thusthefollowingcationicpolymercouldaggregateAuNPsandcausearemarkablechangeinc
5、olor.Accordingtothis,melaminecanbedeterminedbynakedeyesandmeansofabsorbance.Thissensorisselectivefordetectionofmelamineinmilksamplesandhasalimitofdetectionof34nM.Sinceaptamer-modifiedAuNPsismorestabilizedandselectivethanunmodifiedAuNPsinthesensorofme
6、lamine,wehopethatthisdetectionmethod-buildingprocesswillbehelpfulforthedetectionofothersmallmolecules.Keywords：melamine，genetictoxicity，AuNPs，aptamer，detectionVIII萬方數(shù)據(jù)目錄摘要...............................................................................
7、...................................................................VABSTRACT..................................................................................................................................VII圖表清單.....................................
8、..................................................................................................XII縮略表..........................................................................................................................................XIV第一章緒論
