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1、泰山醫(yī)學(xué)院畢業(yè)設(shè)計（論文）題目：蜂膠黃酮抗H2O2誘導(dǎo)PC12細(xì)胞凋亡機(jī)制的研究院（部）系藥學(xué)院所學(xué)專業(yè)藥學(xué)年級、班級完成人姓名指導(dǎo)教師姓名專業(yè)技術(shù)職稱2013年6月18日論文原創(chuàng)性保證書我保證所提交的論文都是自己獨立完成，如有抄襲、剽竊、雷同等現(xiàn)象，愿承擔(dān)相應(yīng)后果，接受學(xué)校的處理。專業(yè)：班級：簽名：20年月日泰山醫(yī)學(xué)院本科畢業(yè)設(shè)計（論文）摘要目的觀察蜂膠黃酮對過氧化氫（H2O2）誘導(dǎo)大鼠腎上腺嗜鉻細(xì)胞瘤細(xì)胞（PC12）凋亡的影響及機(jī)制。方法培養(yǎng)PC12細(xì)胞，取對數(shù)生長期細(xì)胞分為五組，空白對照組、模型組、蜂膠黃酮高、中、低劑量組，劑量分別為200mg/L、100m
2、g/L、50mg/L.藥物預(yù)處理2h后，孵育H2O2（140μmol/L）24h誘導(dǎo)過氧化損傷。TUNEL試劑盒檢測原位細(xì)胞凋亡,流式細(xì)胞儀檢測細(xì)胞內(nèi)活性氧水平以及細(xì)胞周期，ELISA檢測細(xì)胞內(nèi)Caspase-3蛋白含量。結(jié)果TUNEL細(xì)胞凋亡染色顯示,H2O2組與空白對照組比較染色明顯加深,而蜂膠黃酮組染色明顯變淺，說明蜂膠黃酮能對抗H2O2誘導(dǎo)細(xì)胞凋亡；周期結(jié)果顯示H2O2組處于G0/G1細(xì)胞明顯增多，而處于G2/M、S期細(xì)胞明顯減少，細(xì)胞增殖降低，而蜂膠黃酮增加S期細(xì)胞促進(jìn)細(xì)胞增殖；與空白對照組相比H2O2組活性氧水平、細(xì)胞內(nèi)Caspase-3含量明顯增高，
3、而蜂膠黃酮各劑量組活性氧水平降低，Caspase-3含量減少。結(jié)論蜂膠黃酮對H2O2誘發(fā)PC12神經(jīng)細(xì)胞凋亡有顯著的抑制作用，其機(jī)制可能其影響細(xì)胞周期、清除氧自由基、降低凋亡因子Caspase-3有關(guān)。關(guān)鍵詞蜂膠黃酮；PC12細(xì)胞；H2O2;Caspase-3；細(xì)胞凋亡泰山醫(yī)學(xué)院本科畢業(yè)設(shè)計（論文）AbstractObjective:?Toobservetheprotectionof?propolisflavonoids?onratwithinjuriedpheochromocytomacells(PC12)inducedby??hydrogenperoxide(
4、H2O2)?andtoexploreitspossiblemechanism.Methods:???Culture?PC12cells,thecellsin?thelogarithmicgrowthphasewere?dividedintofivegroups,blankcontrolgroup,modelgroup,propolisflavone?ofhigh,mediumandlowdosegroup,thedoseof200mg/L,100mg/L,50mg/L.?After2h0f?Pharmacologicalpreconditioning,thecel
5、ls?wereincubatedwithH2O2(140μmol/L)?for?24h?toinduce?oxidativedamage.?TUNELKit?isusedfor?determiningCellapoptosis,FlowcytometrywasusedtodetecttheintracellularROSlevelandcellcycle,ELISAisusedfordeterminingtheconcentrationofproteinCaspase-3incells.Results:?ApoptosisofTUNELcellsstaining,
6、H2O2groupcomparedwiththecontrolgroup,stainingwasdeepened,andthepropolisflavonegroupwassignificantlylighter,thatpropolisflavonoidscanantagonizetheapoptosisinducedbyH2O2CycleshowedthatH2O2groupinG0/G1cellswereincreased,andintheG2/M,Sphasecellsdecreasedsignificantly,reducedcellproliferat
7、ion,andpropolisflavonoidsincreasedSphasecellspromotingcellproliferation;comparedwiththeblankcontrolgroup,caspase-3inH2O2group,thelevelsofreactiveoxygenspeciesincellsincreasedsignificantly,whilethepropolisflavoneinalldosegroupsdecreasethelevelsofreactiveoxygenspecies,reducedcaspase-3co
8、ntent
