氫水對新生大鼠腦缺血損傷的神經(jīng)保護作用研究碩士學位論文

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1、青島大學碩士學位論文氫水對新生大鼠腦缺血損傷的神經(jīng)保護作用研究姓名：蔡建美申請學位級別：碩士專業(yè)：病理學與病理生理學指導教師：徐珞20090608氫水對新生大鼠腦缺血缺氧損傷的神經(jīng)保護作用研究摘要目的：探討氫生理鹽水對新生大鼠腦缺血缺氧損傷的神經(jīng)保護作用。方法：參照Rice．Vannucci模型，建立新生大鼠腦缺血缺氧模型(HIBD)，采用氯化2，3，5．三苯基四氮唑(2，3，5．triphenyltetrazoliumchloride，TrC)、HE染色和Nissl組織化學染色方法，觀察大鼠皮層和海馬CAl區(qū)細胞凋亡數(shù)；采用Caspase．3／CPP32生物化學法觀察皮層和海

2、馬半胱胺酸蛋白酶蛋白-3(caspase-3)活性變化；通過體重及水迷宮等實驗觀察HS對新生大鼠腦細胞是否具有長期保護效應(yīng)結(jié)果：與缺血對照組比較，腹腔注釋氫生理鹽水可顯著減少新生大鼠皮層和海馬梗死面積(P

3、otectiveeffectsofhydrogensalineinneonatalhypoxia-ischemiaratmodelABSTRACTObjectiveTheaimofthepresentstudywastotestclongtermneuroprotectiveeffectofintraperitonealapplicationofsaturatedhydrogensalineinanestablishedneonatalHImodel．MethodsThemodelusedinthisstudyisbasedontheRice-Vannuccimodel．2,3

4、，5-triphenyltetrazoliumchloride∞C)，HEandNisslstrainingwerepreformedtoevaluatetheeffectofhydrogensaline．(HS)．ImmunohistochemistrywagusedtoobservethenumberofTUNELL-positivecellsindamagedcortexandhippocampusofthedifferentgroups．Theamountsofcaspase·3activitieswereanalyzedbyCaspase-3／CPP32Fluorom

5、etdcAssayKit．At5weeksafterHI，Bodyweight、functiontest、spontaneousactivitytestandMorriswatermazetestwercconductedforthelong-termneurologicalandneurobehavioralfunctions；ResultsReserchontheeffectofhydrogensaline：comparisonwiththeischemiccontrolgroup，hydrogensalineintraperitoneallyCallreducethece

6、rebralinfarctionarea，increasedthesurvivalofnervecells，reducetheCaspase-3activity，andsignificantlyincreasethelong-terrnlearningandmemoryinrats．ConclusionsperitonealadministrationofH2salinereducesbraininjuryafterneonatalHIpossiblybyattenuatingoxidativestress，leadingtoreductionofapoptosisandimp

7、rovementoflongtermneurologicalandneurobehavioralfunctions．Postgraduatestudent：Cai-jiangMei(pathophysiology)Directedby：Prof．LuoXuKeywords：hypoxia-ischemia；hydrogensaline；apoptosis；easpase-3；．學位論文獨創(chuàng)性聲明本人聲明，所呈交的學位論文系本人在導師指導下獨立完成的研究成果。文中依法引用他人的成果，均已做出明