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《煙草青枯病拮抗真菌的篩選、鑒定與活性產(chǎn)物的研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、產(chǎn)物的穩(wěn)定性。發(fā)現(xiàn)菌株3F03的活性產(chǎn)物的熱、pH和紫外穩(wěn)定性較好，經(jīng)蛋白酶K和胰蛋白酶處理抑制率僅分別下降了17．O％、14．5％；菌株7F06的活性產(chǎn)物抗紫外線和蛋白酶的能力較強(qiáng)，酸性環(huán)境下抑菌活性僅稍有下降，活性產(chǎn)物經(jīng)80℃以上高溫處理后抑菌能力基本喪失。關(guān)鍵詞：煙草青枯??；拮抗真菌；活性產(chǎn)物；鑒定：發(fā)酵條件；穩(wěn)定性ScreeningandIdentificationofAntagonisticFungiagainstRalstoniasolanacearumandResearchontheActivePr
2、oductsAbstractTobaccowiltdiseasecausedbyRalatoniasolanacearumisabacterialdisease，widelyoccurredintropicalandsubtropicalzone．IthashappenedandledtoseveredamageinthesouthoftheYangtzeRiverBasinofChina,suchasSichuan，Guangdone,,Fujian，HunanandGuizhouprovince．Yieldl
3、ossoftobaccocanreachupto80％inmostserioustobaccoregion．Traditionalmethodsofchemicalcontrolhavesevereimpactsonecologicalenvironmentfitswellashighcosts，therefore，rotationandmicrobialcontrolwereprovedtobepromisingmethods．Microbialcontrolhastheadvantageofpollution
4、-freeandlong-term，whichcallfundamentallysolvethedrugresidueinthesoil，drugresistanceofpathogensandotherproblems．Itnotonlybenefitstheenvironments，butalsoturnsecologicalresourcesintovirtuouscircle．InordertoprovidethetheoreticalbasisofbiologicalcontrolofRalatonia
5、solanacearum，thisdissertationfocusedparticularlyonstudiesontheactivestrainscreening,identification,optimizationoffermentationconditions，tobaccoseedlingtestinthepresenceoffermentationbroth，andstabilityofproductsofactivestrains，themainresultsaleasfollow．1．Fifly
6、ty-ninefungusstrainswereisolatedfromsoilsamplesintobaccofieldscollectedfromQianjiangdistrict，Chongqing．PaperdiSkmethodWasappliedtoscreenantagonistfungi，and2stains，named3F03and7F06，behavedstronginhibitionactivities．ThediameterofinhibitionzoneWas10．Ommand9．5mm,
7、usingtheconcentrationof250“gcrudeextractperpiecepaperdiskonplate．Repeatedplateantagonismtestshowedthediameterofinhibitionzonehadnoobviousdifference．2．Accordingtothemorphological，cultural，cellularfattyacidandrDNAITSsequenceanalysis，activestrains3F03and7F06were
8、identifiedasAspergillusfumigatusandNeosartoryaaureola，respectively．3．Thegrowthoftobaccoseedlingstreatedwithfermentationbrothofstrains3F03and7F06wasingoodcondition．Comparedwitllcontrols，th
